EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex.
EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. Exp Neurol. 2017 Jul 14;: Authors: Kanemitsu M, Tsupykov O, Potter G, Boitard M, Salmon P, Zgraggen E, Gascon E, Skibo G, Dayer AG, Kiss JZ Abstract Stimulation of endogenous neurogenesis and recruitment of neural progenitors from the subventricular zone (SVZ) neurogenic site may represent a useful strategy to improve regeneration in the ischemic cortex. Here, we tested whether transgenic overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN), the regulator of matrix metalloproteinases (MMPs) expression, in endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) could increase migration towards ischemic injury. For this purpose, we applied a lentivector-mediated gene transfer system. We found that EMMPRIN-transduced progenitors exhibited enhanced MMP-2 activity in vitro and showed improved motility in 3D collagen gel as well as in cortical slices. Using a rat model of neonatal ischemia, we showed that EMMPRIN overexpressing SVZ cells invade the injured cortical tissue more efficiently than controls. Our results suggest that EMMPRIN overexpression could be suitable approach to improve capacities of endogenous or transplanted progenitors to invade the injured cortex. PMID: 28716558 [PubMed - as supplied by publisher]
Newark Beth Israel acted after our report that its transplant program kept a patient alive to improve its metrics, while barely consulting his family.ProPublica
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Pig skin isn ’t just for football these days. Massachusetts General Hospital surgeons have successfully used genetically engineered pig skin for the temporary closure of a burn wound, the first time pig tissue derived from an animal with gene edits has been transplanted directly onto a human wound. The F DA-cleared phase one clinical trial was led by surgeon Dr. Jeremy Goverman of MGH's Sumner Redstone Burn Center. “It is not the trial itself that is so mind-boggling and intriguing to…
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Contributors : Savanne Holster ; Guido J Hooiveld ; Robert J Brummer ; Julia K önigSeries Type : Expression profiling by arrayOrganism : Homo sapiensIn this randomised placebo-controlled trial, irritable bowel syndrome (IBS) patients were treated with faecal material from a healthy donor (n=8, allogenic FMT) or with their own faecal microbiota (n=8, autologous FMT). The faecal transplant was administered by whole colonoscopy into the caecum (30 g of stool in 150 ml sterile saline). Two weeks before the FMT (baseline) as well as two and eight weeks after the FMT, the participants underwent a sigmoidoscopy, and biopsies...
kl Brochot The immunosuppression required for graft tolerance in kidney transplant patients can trigger latent BK polyomavirus (BKPyV) reactivation, and the infection can progress to nephropathy and graft rejection. It has been suggested that pre-transplantation BKPyV serostatus in donors and recipients is a predictive marker for post-transplantation BKPyV replication. The fact that research laboratories have used many different assay techniques to determine BKPyV serostatus complicates these data analysis. Even studies based on the same technique differed in their standard controls choice, the antigenic structure t...
S Rajaian, P Murugavaithianathan, K Krishnamurthy, L MurugasenJournal of Postgraduate Medicine 2019 65(4):247-248
ConclusionsPost-transplant recipients with rpFSGS display a greater symptom burden and experience a more diverse impact than those with non-recurrent pFSGS, highlighting the importance of effective patient monitoring and introducing effective treatments for the prevention and management of pFSGS recurrence.FundingAstellas Pharma Global Development, Inc.
ConclusionsBM-M systemically transplanted, promote a decrease in neuroinflammation and a limited reversion of amyloid pathology. This exploratory study may support the potential of BM-M or microglia-like cell therapy and further illuminates the mechanisms of action associated with such transplants.