SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion.

SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion.
BMB Rep. 2017 Jul 17;:
Authors: Moon H, Cho S, Loh TJ, Jang HN, Liu Y, Choi N, Oh J, Ha J, Zhou J, Cho S, Kim DE, Ye MB, Zheng X, Shen H
Abstract
SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.
PMID: 28712387 [PubMed - as supplied by publisher]

Source: BMB Reports - July 17, 2017 Category: Biochemistry Authors: Moon H, Cho S, Loh TJ, Jang HN, Liu Y, Choi N, Oh J, Ha J, Zhou J, Cho S, Kim DE, Ye MB, Zheng X, Shen H Tags: BMB Rep Source Type: research