D-limonene exhibits superior antihyperalgesic effects in a β-cyclodextrin-complexed form in chronic musculoskeletal pain reducing Fos protein expression on spinal cord in mice

Publication date: 1 September 2017 Source:Neuroscience, Volume 358 Author(s): Heitor G. Araújo-Filho, Erik W.M. Pereira, Marília M. Rezende, Paula P. Menezes, Adriano A.S. Araújo, Rosana S.S. Barreto, Anita O.B.P.B. Martins, Thaís R. Albuquerque, Bruno A.F. Silva, Isabel S. Alcantara, Henrique D.M. Coutinho, Irwin R.A. Menezes, Lucindo J. Quintans-Júnior, Jullyana S.S. Quintans Chronic musculoskeletal pain is one of the main symptoms found in Fibromyalgia with unclear etiology and limited pharmacological treatment. The aim of this study was to complex LIM in β-cyclodextrin (LIM-βCD) and then evaluate its antihyperalgesic effect in an animal model of chronic musculoskeletal pain. Differential scanning calorimetry and scanning electron microscopy was used for the characterization of the inclusion complex. Male Swiss mice were used for experimental procedures where mechanical hyperalgesia, thermal hyperalgesia, muscular strength, Fos immunofluorescence was studied after induction of hyperalgesia. Mechanism of action was also investigated through tail flick test and capsaicin-induced nociception. Endothermic events and morphological changes showed that the slurry complex method was the best method for the complexation. After induction of hyperalgesia, the oral administration of LIM-βCD (50mg/kg) significantly increased the paw withdrawal threshold compared to uncomplexed limonene. Fos immunofluorescence showed that both compounds significantly decreased th...
Source: Neuroscience - Category: Neuroscience Source Type: research