Cag Pathogenicity Island-Dependent Up-Regulation Of Matrix Metalloproteinase-7 In Infected Patients With Helicobacter Pylori.

Cag Pathogenicity Island-Dependent Up-Regulation Of Matrix Metalloproteinase-7 In Infected Patients With Helicobacter Pylori. J Immunoassay Immunochem. 2017 Jul 12;: Authors: Sadeghiani M, Bagheri N, Shahi H, Reiisi S, Rahimian G, Rashidi R, Mahsa M, Shafigh M, Salimi E, Rafieian-Kopaei M, Hashemzadeh-Chaleshtori M, Shirzad H Abstract Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a family of secreted, zinc-dependent enzymes collectively capable of degradation of extracellular matrix (ECM) and key player in various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of virulence factors, such as vacA (vacuolating cytotoxin A) and cagA (cytotoxin-associated gene) in H. pylori-infected patients upon the MMP-7 mRNA mucosal levels. We also determined correlation between mucosal MMP-7 mRNA levels and types of disease. Total RNA was extracted from gastric biopsies of 50 H. pylori-infected patients and 50 uninfected individuals. Mucosal MMP-7 mRNA expression level in H. pylori-infected and non-infected gastric biopsies was determined by real time-PCR. Presences of cagA and vacA virulence factors were evaluated using PCR. MMP-7 expression was significantly higher in biopsies of patients infected with H .pylori compared to uninfected i...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research