Genome-wide Mapping of the Nucleosome Landscape by Micrococcal Nuclease and Chemical Mapping.

Genome-wide Mapping of the Nucleosome Landscape by Micrococcal Nuclease and Chemical Mapping.
Trends Genet. 2017 Jul 07;:
Authors: Voong LN, Xi L, Wang JP, Wang X
Abstract
Nucleosomes regulate the transcription output of the genome by occluding the underlying DNA sequences from DNA-binding proteins that must act on it. Knowledge of the precise locations of nucleosomes in the genome is thus essential towards understanding how transcription is regulated. Current nucleosome-mapping strategies involve digesting chromatin with nucleases or chemical cleavage followed by high-throughput sequencing. In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemical cleavage strategy, with discussion on the important insights each has uncovered about the role of nucleosomes in shaping transcriptional processes.
PMID: 28693826 [PubMed - as supplied by publisher]

Source: Trends in Genetics : TIG - July 7, 2017 Category: Genetics & Stem Cells Authors: Voong LN, Xi L, Wang JP, Wang X Tags: Trends Genet Source Type: research