Efficient dual siRNA and drug delivery using engineered lipoproteoplexes.

Efficient dual siRNA and drug delivery using engineered lipoproteoplexes.
Biomacromolecules. 2017 Jul 07;:
Authors: Liu CF, Chen R, Frezzo JA, Katyal P, Hill LK, Yin L, Srivastava N, More HT, Renfrew PD, Bonneau R, Montclare JK
Abstract
An engineered supercharged coiled-coil protein (CSP) and the cationic transfection reagent Lipofectamine 2000 are combined to form a lipoproteoplex for the purpose of dual delivery of siRNA and doxorubicin. CSP, bearing an external positive charge and axial hydrophobic pore, demonstrates the ability to condense siRNA and encapsulate the small molecule chemotherapeutic, doxorubicin. The lipoproteoplex demonstrates improved doxorubicin loading relative to Lipofectamine 2000. Furthermore, it induces effective transfection of GAPDH (60% knockdown) in MCF-7 breast cancer cells with efficiencies comparing favorably to Lipofectamine 2000. When the lipoproteoplex is loaded with doxorubicin, the improved doxorubicin loading (~40 µg Dox/mg CSP) results in a substantial decrease in MCF-7 cell viability.
PMID: 28686014 [PubMed - as supplied by publisher]

Source: Biomacromolecules - July 7, 2017 Category: Biochemistry Authors: Liu CF, Chen R, Frezzo JA, Katyal P, Hill LK, Yin L, Srivastava N, More HT, Renfrew PD, Bonneau R, Montclare JK Tags: Biomacromolecules Source Type: research