Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis

Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3–Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space group P212121, with unit-cell parameters a = 51.6, b = 86.9, c = 88.7 Å.

Source: Acta Crystallographica Section F - June 18, 2014 Category: Biochemistry Authors: Zheng, S.Ye, K. Tags: ribosome synthesis Imp3 Mpp10 90S pre-ribosome Saccharomyces cerevisiae crystallization communications Source Type: research