Structure of d(CCCCGGTACCGGGG)2 at 1.65 Å resolution

The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG)2 has previously been reported as an A-type double helix at a resolution of 2.5 Å in space group P41. Here, the structure of this sequence was determined at a significantly higher resolution of 1.65 Å in space group P41212. The differences in crystal packing between the former and latter are described. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex that spans more than one full turn of the A-helix. This structure allowed the unambiguous identification of solvent interactions.

Source: Acta Crystallographica Section F - June 18, 2014 Category: Biochemistry Authors: Purushothaman, M.Varghese, A.Mandal, P.K.Gautham, N. Tags: A-DNA tetradecanucleotide right handed double helix structural communications Source Type: research