Comparison of waterborne and intraperitoneal exposure to fipronil in the Caspian white fish (Rutilus frisii) on acute toxicity and histopathology

Publication date: Available online 23 June 2017 Source:Toxicology Reports Author(s): Rashid Alijani Ardeshir, Hossein Zolgharnein, Abdol-Ali Movahedinia, Negin Salamat, Ebrahim Zabihi Fipronil is an effective insecticide widely used in agriculture with potential ecotoxicological consequences. The median lethal dose (LD50) and concentration (LC50) of fipronil in 16.3g Caspian white fish, Rutilus frisii kutum fingerlings were determined. To determine the LD50, a total of 133 fish were assigned to 19 tanks (7 fish/tank) including one control and 6 treatment groups (300, 450, 550, 650, 750, 850mg/kg). Fish were injected intraperitoneally and monitored at 96h. The LD50 of fipronil was 632mg/kg suggesting it was slightly toxic to the Caspian white fish. To determine LC50, 114 fish were assigned to 19 tanks (6 fish/tank) including one control and 6 treatment groups (300, 400, 500, 600, 700, 800μg/L). The LC50 of fipronil was 572μg/L, which was highly toxic to the fish. The degree of tissue change (DTC) in vital organs from moribund fish exposed via waterborne exposure showed severe damage (DTC: 71±52 for 700μg/L) in the gill, including aneurisms, extensive fusion and necrosis. The fish exposed through the intraperitoneal route seemed to have severe lesions (DTC: 66±50 for 750mg/kg) in the kidney, involving hemorrhage, tubular degeneration and necrosis. The liver had no significant differences in DTC values between the two routes and showed pyknosis and sinusoid dilation....
Source: Toxicology Reports - Category: Toxicology Source Type: research