Substitution of strontium and boron into hydroxyapatite crystals: Effect on physicochemical properties and biocompatibility with human Wharton-Jelly stem cells.
Substitution of strontium and boron into hydroxyapatite crystals: Effect on physicochemical properties and biocompatibility with human Wharton-Jelly stem cells.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 01;79:638-646
Authors: Kolmas J, Velard F, Jaguszewska A, Lemaire F, Kerdjoudj H, Gangloff SC, Kaflak A
Abstract
Hydroxyapatite (HA) enriched with strontium and boron ions was synthesized using two different methods: the precipitation method (Sr,B-HAw) and the dry method (Sr,B-HAd). Additionally, for the sake of comparison, the "pure" unsubstituted HA was prepared together with HAs substituted only with one type of a foreign ion. The obtained materials were subjected to physicochemical analysis with the use of various analytical methods, such as powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), inductively coupled plasma optical emission spectroscopy (ICP-OES), Fourier transform infrared spectroscopy (FT-IR) and solid-state proton nuclear magnetic resonance ((1)H ssNMR). All the obtained materials were also biologically tested for their potential cytotoxicity. The obtained materials (Sr,B-HAw and Sr,B-HAd) were homogeneous and respectively showed nano- and microcrystal apatitic structures. The simultaneous introduction of Sr(2+) and BO3(3-) ions turned out to be more effective in respect of the dry method. Of importance, doped materials obtained using both synthesis routes have been demonstrated to be biocompati...
Source: Appl Human Sci - Category: Physiology Authors: Kolmas J, Velard F, Jaguszewska A, Lemaire F, Kerdjoudj H, Gangloff SC, Kaflak A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research