How to speed up the polymerase chain reaction

Publication date: Available online 20 June 2017 Source:Biomolecular Detection and Quantification Author(s): Stephen A. Bustin Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research