A Method for Measuring Activity Difference of Phenolic and Non-Phenolic Groups through ABTS Radical Cation.

A Method for Measuring Activity Difference of Phenolic and Non-Phenolic Groups through ABTS Radical Cation.
Comb Chem High Throughput Screen. 2014 Jun 5;
Authors: Masood N, Luqman S
Abstract
We have optimized a 2, 2`azino-bis-(3-ethyl-benzothiazoline-6-sulphonate) radical cation assay procedure to be performed in 96 well plates and evaluated the scavenging potential of phenolic and non-phenolic group of molecules with respect to control. Behaviour of molecules with ABTS radical cation has been studied and the influence of structure and concentration on the bleaching of the stable bluish green ABTS radical cation complex formation is depicted. Experimentally, it was observed that phenolic molecules showed higher reactivity with ABTS radical than non-phenolic molecules because of resonance and hyperconjugation effect.
PMID: 24910258 [PubMed - as supplied by publisher]

Source: Combinatorial Chemistry and High Throughput Screening - June 5, 2014 Category: Chemistry Authors: Masood N, Luqman S Tags: Comb Chem High Throughput Screen Source Type: research