Osteocyte Regulation of Receptor Activator of NF- κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis. Am J Pathol. 2017 Jun 12;: Authors: El Khassawna T, Merboth F, Malhan D, Böcker W, Daghma DES, Stoetzel S, Kern S, Hassan F, Rosenbaum D, Langenstein J, Bauer N, Schlagenhauf A, Rösen-Wolff A, Schulze F, Rupp M, Hose D, Secklinger A, Ignatius A, Wilke HJ, Lips KS, Heiss C Abstract Osteoporosis induction in a sheep model by steroid administration combined with ovariectomy recapitulates decreased bone formation and substandard matrix mineralization in patients. Recently, the role of osteocytes has been frequently addressed, with focus on their role in osteoclastogenesis. However, the quantification of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) signaling in osteocytes was not studied in sheep. The current study reproduced the sheep model of osteoporosis to study the RANKL/OPG ratio correlation to the method of osteoporosis induction. We investigated the induction of osteoporosis after 8 months using 31 female merino land sheep divided into four groups: control, ovariectomy, ovariectomy with dietary limitation, and ovariectomy with dietary limitation and steroid injection. In accordance to previous reports, the present study showed trabecular thinning, higher numbers of apoptotic osteocytes, and imbalanced metabolism, leading to defective mineralization. The global RANKL/OPG ratio in the spine after 8...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research