Immunohistochemical Profiling of Corneas With Fuchs Endothelial Corneal Dystrophy

Purpose: Fuchs endothelial corneal dystrophy (FECD) is the leading indication for endothelial keratoplasty. Further insight into its pathophysiology is needed to develop alternative therapies. Methods: Sixteen genes from a previous microarray expression experiment (FECD vs. normal) were validated using immunohistochemistry on paraffin-embedded corneas (n = 6 FECD, n = 6 normal). The results were quantified manually and semiautomatically. Results: A higher percentage of corneal endothelial cells stained for alpha–smooth muscle actin (αSMA), cytokeratin 7, and superoxide dismutase 3 in FECD versus normal [odds ratios (ORs) of 60.90, 41.70, and 15.16, respectively, P
Source: Cornea - Category: Opthalmology Tags: Basic Investigation Source Type: research