Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT

Publication date: Available online 7 June 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jian-You Liao, Jue Wu, Yan-Jie Wang, Jie-Hua He, Wei-Xi Deng, KaiShun Hu, Yu-Chan Zhang, Yin Zhang, Haiyan Yan, Dan-Lan Wang, Qiang Liu, Mu-Sheng Zeng, H. Phillip Koeffler, Erwei Song, Dong Yin Several studies have shown that long non-coding RNAs (lncRNAs) may play an essential role in Epithelial-Mesenchymal Transition (EMT), which is an important step in tumor metastasis; however, little is known about the global change of lncRNA transcriptome during EMT. To investigate how lncRNA transcriptome alterations contribute to EMT progression regulation, we deep-sequenced the whole-transcriptome of MCF10A as the cells underwent TGF-β-induced EMT. Results Deep-sequencing results showed that the long RNA transcriptome of MCF10A had undergone global changes as early as 8h after treatment with TGF-β. The expression of 3,403 known and novel lncRNAs, and 570 known and novel circRNAs were altered during EMT. To identify the key lncRNA-regulator, we constructed the co-expression network and found all junction nodes in the network are lncRNAs. One junction node, RP6-65G23.5, was further verified as a key regulator of EMT. Intriguingly, we identified 216 clusters containing lncRNAs which were located in “gene desert” regions. The expressions of all lncRNAs in these clusters changed concurrently during EMT, strongly suggesting that these cluste...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research