Isolation and characterization of Listeria species from rodents in natural environments in China

Isolation and characterization of Listeria species from rodents in natural environments in China Emerging Microbes &Infections 6, e44 (June 2017). doi:10.1038/emi.2017.28 Authors: Yan Wang, Liang Lu, Ruiting Lan, Joelle K Salazar, Jingli Liu, Jianguo Xu &Changyun Ye
Source: Emerging Microbes and Infections - Category: Infectious Diseases Authors: Tags: antimicrobial resistance Listeria molecular characteristic natural environments rodents Source Type: research

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Listeria monocytogenes is a Gram-positive bacterium that can be found in a broad range of environments, including soil, food, animals, and humans. L. monocytogenes can cause a foodborne disease manifesting as sepsis and meningo-encephalitis. To evaluate signals of selection within the core genome of neuroinvasive L. monocytogenes strains, we sequenced 122 L. monocytogenes strains from cerebrospinal fluid (CSF) of Dutch meningitis patients and performed a genome-wide analysis using Tajima’s D and ω (dN/dS). We also evaluated the residual variation intolerance score (RVIS), a computationally less demanding method...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
koh Disease outbreaks caused by the ingestion of contaminated vegetables and fruits pose a significant problem to human health. The sources of contamination of these food products at the preharvest level of agricultural production, most importantly, agricultural soil and irrigation water, serve as potential reservoirs of some clinically significant foodborne pathogenic bacteria. These clinically important bacteria include: Klebsiella spp., Salmonella spp., Citrobacter spp., Shigella spp., Enterobacter spp., Listeria monocytogenes and pathogenic E. coli (and E. coli O157:H7) all of which have the potential to cause dise...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
Most environmental biofilms contain a variety of species. These species can establish cooperative and competitive interactions, possibly resulting in an increase or a decrease in antimicrobial resistance. Therefore, results obtained following inactivation of single-species biofilms by means of different technologies (e.g., Cold Atmospheric Plasma, CAP) should be validated for multi-species biofilms. First, a strongly adherent and mature Listeria monocytogenes and S. Typhimurium dual-species biofilm was developed by altering different incubation conditions, i.e., growth medium, incubation temperature, inoculum ratio of L. m...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The objective of this study was to identify a surrogate of L. monocytogenes to validate the sanitation efficiency of fresh apples. We evaluated and compared the behavior of Listeria innocua, Enterococcus faecium, Pediococcus acidilactici and Lactobacillus plantarum, to that of L. monocytogenes on apples regarding attachment, survival during storage, and resistance to various sanitizer washes. L. monocytogenes, E. faecium and L. innocua persisted on fresh apple surfaces at 6.4-6.5 Log10 CFU/apple for 48 h at room temperature, while L. plantarum and P. acidilactici slightly decreased. All the selected surrogate candidates de...
Source: Food Control - Category: Food Science Source Type: research
In this study, we targeted a highly conserved region of these proteins by an antisense sequence that was covalently conjugated to the cell penetrating peptides (CPP) to overcome the challenging delivery barriers. Then, we evaluated the efficiency of this construct in vitro. We also assessed the antigenicity, cytotoxicity, and probability of apoptosis induction by this construct.The studied CPP-PNA inhibited bacterial growth and suppressed the mRNA expression of internalins in a dose-dependent manner. In addition, at all studied concentrations, CPP-PNA significantly reduced the invasion rate of L. monocytogenes in the exami...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Abstract Bacterial RecA plays an important role in the evaluation of antibiotic resistance via stress-induced DNA repair mechanism; SOS response. Accordingly, RecA became an important therapeutic target against antimicrobial resistance. Small molecule inhibitors of RecA may prevent adaptation of antibiotic resistance mutations and the emergence of antimicrobial resistance. In our study, we observed that phenolic compound p-Coumaric acid as potent RecA inhibitor. It inhibited RecA driven biochemical activities in vitro such as ssDNA binding, strand exchange, ATP hydrolysis and RecA coprotease activity of E.&nb...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research
This study describes TCS/ABC transporter modules characterized in Listeria monocytogenes EGD-e. It consists of the VirSR TCS and the VirAB and AnrAB ABC transporters. Our results showed that this system is involved in nisin and bacitracin resistance, as well as cephalosporins, ethidium bromide (EtBr) and benzalkonium chloride (BC) resistance. In this system, VirAB is only responsible for antimicrobial sensing and signalling by VirSR, while AnrAB contributes to transport antimicrobial. Interestingly, VirAB itself, instead of the VirAB-VirSR-AnrAB system, contributes to kanamycin and tetracycline resistance. PMID: 31399...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Foodborne diseases (FBDs) are infections of the gastrointestinal tract caused by foodborne pathogens (FBPs) such as bacteria (Salmonella, Listeria monocytogenes and Shiga toxin-producing E. coli (STEC)) and several viruses, but also parasites and some fungi. Artificial intelligence (AI) and its sub-discipline machine learning (ML) are re-emerging and gaining an ever increasing popularity in the scientific community and industry, and could lead to actionable knowledge in diverse ranges of sectors including epidemiological investigations of FBD outbreaks and antimicrobial resistance (AMR). As genotyping using whole-genome se...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Contamination of food during processing is recognized as main transmission route of Listeria (L.) monocytogenes To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens due to widespread biocide usage.In our study, 93 L. monocytogenes isolates from German food production facilities were i) tested for biocide and antibiotic susceptibility using broth microdilution assays, ii) analyzed for links between reduced biocide susceptibility and antibiotic resistanc...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
In conclusion, free curcumin showed potent antibacterial effects; however, the nanoencapsulated form was able to minimize the effects caused by L. monocytogenes regarding tissue injury, changes on enzymes of the energetic metabolism, in addition to an antioxidant effect against lipoperoxidation.Graphical abstract
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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