GSE97618 Opposing roles of Toll-like receptor and cytosolic DNA-STING signaling pathways for Staphylococcus aureus cutaneous host defense

Contributors : Philip O Scumpia ; Joshua S NormanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSuccessful host defense against pathogens requires innate immune recognition of the correct pathogen associated molecular patterns (PAMPs) by pathogen recognition receptors (PRRs) to trigger the appropriate gene program tailored to the pathogen. While many PRR pathways have been shown to contribute to the innate immune response to specific pathogens, the relative importance of each pathway for the complete transcriptional program elicited has not been examined in detail. Herein, we used RNA-sequencing with wildtype and mutant macrophages to delineate the innate immune pathways responsible for the early transcriptional response to Staphylococcus aureus, a ubiquitous microorganism that can activate a wide variety of PRRs. Unexpectedly, only two PRR pathways – the Toll-like receptor (TLR) and Stimulator of Interferon Gene (STING) pathways - were identified as dominant regulators of approximately 95% of the genes that were potently induced within the first four hours of macrophage infection with live S. aureus. TLR signaling predominantly activated an inflammatory program, STING signaling activated an antiviral/type I interferon response, and both pathways contributed to a program linking innate and adaptive immunity. Only a small number of genes were induced in the absence of TLR or STING signaling, and these genes possessed a strong hypox...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

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Publication date: 15 May 2020Source: Journal of Alloys and Compounds, Volume 823Author(s): P. Sochacka, A. Miklaszewski, M. Jurczyk, P. Pecyna, M. Ratajczak, M. Gajecka, M.U. JurczykAbstractIn the present study, the crystal structure, microstructure, mechanical and corrosion properties of bulk Ti31MoxHA composites (x = 0, 2.5, 5 and 10 wt %) were investigated. The sintering of Ti31MoxHA powders led to the formation of a bulk composite with grain size of approx. 1 μm. All these composites have elastic modulus lower than CP microcrystalline α-Ti, and their hardness is two times higher. The ultraf...
Source: Journal of Alloys and Compounds - Category: Chemistry Source Type: research
Publication date: Available online 18 January 2020Source: Toxicology ReportsAuthor(s): Afaf M. Weli, Hanady S. Al-Saadi, Rouqaya S. Al-Fudhaili, Amzad Hossain, Zabida Binti Putit, Mahmood K. JasimAbstractMedicinal plant as herbal medicine is widely used to cure infectious diseases. Rubus fruticosus L (R. fruticosus) has been studied for its antimicrobial and cytotoxic activities. Different polarity leave extracts were prepared by using hexane, chloroform, ethyl acetate and hydro alcoholic solvents. Agar diffusion method has been used to assess the antibacterial activity against two gram-positive Enterococcus faecalis and S...
Source: Toxicology Reports - Category: Toxicology Source Type: research
This study provides strong evidence that following a healthy lifestyle can substantially extend the years a person lives disease-free." Commentary on Recent Evidence for Cognitive Decline to Precede Amyloid Aggregation in Alzheimer's Disease https://www.fightaging.org/archives/2020/01/commentary-on-recent-evidence-for-cognitive-decline-to-precede-amyloid-aggregation-in-alzheimers-disease/ I can't say that I think the data presented in the research noted here merits quite the degree of the attention that it has been given in the popular science press. It is interesting, but not compelling if its role...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Mice cured of MRSA, raising hopes of treating antibiotic-resistant bacteriaA compound made by cannabis plants has been found to wipe out drug-resistant bacteria, raising hopes of a new weapon in the fight against superbugs.Scientists screened five cannabis compounds for their antibiotic properties and found that one, cannabigerol (CBG), was particularly potent at killing methicillin-resistant Staphylococcus aureus (MRSA), one of the most common hospital superbugs.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Drug resistance Antibiotics Medical research Cannabis Science UK news Source Type: news
This study underscores the capacity of human DC to discriminate between live and inactivated S. aureus and, further, indicates that DC may represent a valuable experimental setting to test different inactivation methods with regard to the retention of S. aureus immunoregulatory properties. These and further insights may be useful for the development of novel therapeutic and prophylactic anti-S. aureus vaccine strategies.
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Jassy Mary S. Lazarte1, Kim D. Thompson2 and Tae Sung Jung1* 1Laboratory of Aquatic Animal Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea 2Moredun Research Institute, Pentlands Science Park, Penicuik, United Kingdom Teleost fish, as with other vertebrates, rely on their innate immune system as a first line of defense against invading pathogens. A very important characteristic of the innate immune response is its ability to recognize conserved molecular structures, such as viral dsRNA and ssRNA. Mda5 is one of the three pattern recognition receptors (PRRs) that recogn...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Authors: Placinta IA, España-Gregori E, Rodrigo-Hernández A, Martínez-Rubio C, Safont-Albert J, Bort-Martí MÁ Abstract An 80 year-old woman with no relevant medical history, consulted for worsening of right palpebral itching and pain after an insect bite. Her eyelids had areas of laceration due to scratching, which rapidly progressed to fibrinoid necrosis over the first 24hours. Lesions were cultivated, revealing Streptococcus pyogenes and Staphylococcus aureus. The patient was admitted to hospital with the diagnosis of periorbital necrotising fasciitis, in order to receive treatm...
Source: Archivos de la Sociedad Espanola de Oftalmologia - Category: Opthalmology Tags: Arch Soc Esp Oftalmol Source Type: research
​Some medical conditions have signs and symptoms that significantly overlap, making a diagnosis a little more difficult. Epididymitis, testicular torsion, and torsion of the testicular appendage are examples, but orbital and preseptal cellulitis are others that can cause significant diagnostic confusion.Both conditions are more common in children than in adults, and preseptal or periorbital cellulitis is more common in children under 5. The preseptal and orbital spaces are separated by only a thin membranous septum that originates in the orbital periosteum and inserts into the tarsal plates. It is only this thin septum t...
Source: M2E Too! Mellick's Multimedia EduBlog - Category: Emergency Medicine Tags: Blog Posts Source Type: blogs
CONCLUSION: This case illustrates an anecdotal example of very late CP infection, due to an unpredictable accident. Due to lack of consensus on risk factors and on conservative or surgical strategy in case of graft infection, we aimed to share our surgical experience. PMID: 28868201 [PubMed]
Source: Surgical Neurology International - Category: Neurosurgery Tags: Surg Neurol Int Source Type: research
This study examined the possibility that c-di-AMP released during S. aureus biofilm growth polarizes macrophages toward an anti-inflammatory phenotype via type I interferon signaling. DacA, the enzyme responsible for c-di-AMP synthesis in S. aureus, was highly expressed during biofilm growth, and 30 to 50% of total c-di-AMP produced from S. aureus biofilm was released extracellularly due to autolytic activity. S. aureus biofilm c-di-AMP release induced macrophage type I IFN expression via a STING-dependent pathway and promoted S. aureus intracellular survival in macrophages. These findings identify c-di-AMP as another mech...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host Response and Inflammation Source Type: research
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