ABCB1 gene polymorphism associated with clinical factors can predict drug-resistant tuberculosis

Polymorphism in ABCB1 gene encoding P-glycoprotein, a transmembrane drug efflux pump, contributes to drug resistance and have been widely studied, however, their association with rifampicin and ethambutol resistance in tuberculosis patients are still unclear. Genotype/allele/haplotype frequencies in c.1236C>T (rs1128503), c.2677G>T/A (rs2032582) and c.3435C>T (rs1045642) were obtained from 212 patients, of these, 80 patients with rifampicin and/or ethambutol resistance were selected as case group and 132 control-group through the result of their culture and drugs sensitive test. Patients aging ABCB1 polymorphisms were determined using a polymerase chain reaction, direct sequencing approach and restriction fragment length polymorphism. A nomogram was constructed to simulate a combined prediction of probability to anti-tuberculosis drug resistance, genotype c.1236C> T (rs1128503) (p= 0.02), clinical form (p= 0.03), previous treatment (p=0.01) and skin color (p = 0.03), have a chance of up to 90% of developing anti-tuberculosis drug resistance. Considering genotype analyzes, CT (rs1128503) demonstrated an increased chance to anti-tuberculosis drugs resistance (odds ratio: 2.34, p= 0.02), while the analyses for ethambutol resistance revealed an association of rare allele "A" (rs2032582) (odds ratio: 12.91, p=0.01), the haplotype TTC (odds ratio: 5.83, p= 0.05) and any haplotype containing the rare allele "A" (odds ratio: 7.17, p=0.04). The ABCB1 gene polymorphisms...
Source: Clinical Science - Category: Biomedical Science Authors: Tags: PublishAheadOfPrint Source Type: research