Effect of biocontrol agent Bacillus amyloliquefaciens SN16-1 and plant pathogen Fusarium oxysporum on tomato rhizosphere bacterial community composition

Publication date: Available online 1 June 2017 Source:Biological Control Author(s): Tingting Wan, Huihui Zhao, Wei Wang The fungus Fusarium oxysporum causes tomato plant wilt, which lead to economic losses worldwide. Bacillus spp. has been widely used as a biological control agent against tomato wilt; in a previous study, Bacillus amyloliquefaciens SN16-1 showed great potential for controlling F. oxysporum f. sp. lycopersici (FOL). However, little is known about the effects of SN16-1 and FOL on tomato rhizosphere bacterial community composition. To address this issue, the present study investigated changes in community diversity following SN16-1 and FOL treatment by Illumina MiSeq sequencing of the 16S rRNA V4 region. The sequences were used to establish operational taxonomic units and were classified into 22 phyla and 323 genera, respectively. Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, and Bacteroidetes were the most highly represented phyla in each sample. Pseudomonas and Massilia were more abundant in the SN16-1 group, whereas Chthoniobacter and Haloferula were more highly represented in the FOL group. Diversity indices and principal component analysis indicated that treatment with SN16-1 and FOL had transient effects on the rhizosphere bacterial community. In summary, these results combined with the functional analysis of the changed genera provide safe and effective measure associated with using B. amyloliquefaciens SN16-1 for the control of to...

Source: Biological Control - June 1, 2017 Category: Biology Source Type: research