In  vitro effects of Zinc in soluble and homeopathic formulations on macrophages and astrocytes.

In vitro effects of Zinc in soluble and homeopathic formulations on macrophages and astrocytes. Homeopathy. 2017 May;106(2):103-113 Authors: Bonafini C, Marzotto M, Bellavite P Abstract Zinc is an important metal in body homeostasis. Zinc in soluble form (Zn(2+)) and homeopathic Zincum metallicum were tested in macrophages and astrocytes in order to investigate its potential toxic or therapeutic effects. We evaluated cell viability (WST assay), cytokine production such as tumour necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) by enzyme-linked immunosorbent assay (ELISA) and nitric oxide release by Griess reaction. The effect of zinc-depletion and high zinc pre-treatments on the cell adaptation capability was also investigated. In THP-1 macrophage cell line and in human primary macrophages, Zn(2+) at sub-toxic doses (30 μM) caused stimulation of TNF-α and IL-10 with different dynamics reaching the maximum peak at the zinc concentration 100 μM, before the cell death. Highest doses (300 μM) impaired dramatically cell vitality. Similar effects on cell viability were obtained also in C6 astrocytes, where Zn(2+) slightly increased the nitric oxide release only in cells activated by one of the pro-inflammatory stimuli used in our cellular model (interferon gamma plus TNF-α). Zinc depletion markedly reduced IL-10 production and cell viability. Zincum metallicum did not cause toxicity in any cell type and sh...
Source: Homeopathy - Category: Complementary Medicine Authors: Tags: Homeopathy Source Type: research

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