Improvement of ELISA procedures through simultaneous addition of antigen and detection antibody and elimination of washing steps.

Improvement of ELISA procedures through simultaneous addition of antigen and detection antibody and elimination of washing steps.
J Immunoassay Immunochem. 2017 May 24;:
Authors: Gurevich V, Kotharu P, McCann K, Bertolini J
Abstract
The enzyme-linked immunosorbent assay (ELISA) is extensively used for measurement of proteins utilised for various research and diagnostic purposes in the biological disciplines. However it is a labour intensive and lengthy procedure due to a number of incubation and washing steps required for performing the assay. The ELISA procedure in the current study has been simplified through the simultaneous addition of antigen and detection antibody and elimination of washing steps. This resulted in a decreased time required to perform the procedure and without affecting assay capability. This approach offers the possibility of increasing ELISA productivity in low throughput laboratories without the need for alternative analytical platforms which would require significant assay redevelopment and capital expense.
PMID: 28537469 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - May 26, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research