Genetically Encoded Calcium Indicators as Probes to Assess the Role of Calcium Channels in Disease and for High-Throughput Drug Discovery.
Genetically Encoded Calcium Indicators as Probes to Assess the Role of Calcium Channels in Disease and for High-Throughput Drug Discovery. Adv Pharmacol. 2017;79:141-171 Authors: Bassett JJ, Monteith GR Abstract The calcium ion (Ca(2+)) is an important signaling molecule implicated in many cellular processes, and the remodeling of Ca(2+) homeostasis is a feature of a variety of pathologies. Typical methods to assess Ca(2+) signaling in cells often employ small molecule fluorescent dyes, which are sometimes poorly suited to certain applications such as assessment of cellular processes, which occur over long periods (hours or days) or in vivo experiments. Genetically encoded calcium indicators are a set of tools available for the measurement of Ca(2+) changes in the cytosol and subcellular compartments, which circumvent some of the inherent limitations of small molecule Ca(2+) probes. Recent advances in genetically encoded calcium sensors have greatly increased their ability to provide reliable monitoring of Ca(2+) changes in mammalian cells. New genetically encoded calcium indicators have diverse options in terms of targeting, Ca(2+) affinity and fluorescence spectra, and this will further enhance their potential use in high-throughput drug discovery and other assays. This review will outline the methods available for Ca(2+) measurement in cells, with a focus on genetically encoded calcium sensors. How these sensors will improve our understanding of the d...
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Readers who found these articles interesting may also like to read these papers that can be found in recent issues of our sister publications, Seminars in Thoracic and Cardiovascular Surgery and Operative Techniques in Thoracic and Cardiovascular Surgery.
Re: Yang CJ, Kumar A, Gulack BC, Mulvihill MS, Hartwig MG, Wang X, et al. Long-term outcomes after lobectomy for non–small cell lung cancer when unsuspected pN2 disease is found: A National Cancer Data Base analysis. J Thoracic Cardiovasc Surg. 2016;151:1380-8.
Dr M. Jacobs (Baltimore, Md). The Norwood procedure, the most commonly performed open operation in the neonatal age group, was developed approximately 40 years ago by Dr William Norwood. This operation has probably been the subject of as many or more investigations or reports than any other operation for congenital heart disease, yet Dr Mascio and colleagues stated accurately in their article that the principles of the Norwood operation remain esse ntially the same today as when Norwood first conceived it.