Quality control of the slides by Kato-Katz method for the parasitological diagnosis of schistosomiasis infection by Schistosoma mansoni

Conclusion: This study provides the laboratory which performs the diagnosis of schistosomiasis using the Kato-Katz method with parameters to implement a diagnostic service that can be evaluated internally and externally. The establishment of a quality protocol enables the comparison of data and the identification of failures in the operational procedure, which can be corrected by training personnel and taking actions for the problems identified.RESUMO Introdu ção: O Kato-Katz é o método laboratorial adotado pelo Ministério da Saúde (MS) e pela Organização Mundial da Saúde (OMS) como padrão-ouro para o diagnóstico da infecção humana pelo Schistosoma mansoni, sendo uma ferramenta de relevância clínica e epidemiológica, visto que permite cla ssificar a carga parasitária do indivíduo infectado pelo cálculo de ovos por grama de fezes (OPG). Essa classificação pode também ser utilizada para estimar a intensidade da infecção nas comunidades, mensurar o impacto de medidas de controle da doença bem como estabelecer parâmetros de qua lidade para a leitura das lâminas. Objetivo: Descrever os procedimentos laboratoriais corretos para o diagnóstico parasitológico da infecção pelo S. mansoni pelo método Kato-Katz a partir do protocolo de controle de qualidade estabelecido ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - Category: Pathology Source Type: research

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Publication date: Available online 15 July 2019Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Cora Delling, Denny Böttcher, Vivien Schiffbauer, Andreas Bernhard, Ronald SchmäschkeAbstractThe cestode Taenia crassiceps parasitizes in the intestine of domestic and wild carnivores, especially in red foxes. Usually, the metacestode stage, also known as Cysticercus longicollis, is located in muscles, peritoneal and pleural cavity of wild rodents. In this case, larval stages were found in a female Cape fur seal, which lived in a German zoo since June 1998. In January 2019, the animal pr...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Authors: Yoo Jung L, Lee YH, Chung DH, Shin JW PMID: 31303087 [PubMed - as supplied by publisher]
Source: Journal of Obstetrics and Gynaecology - Category: OBGYN Tags: J Obstet Gynaecol Source Type: research
A concept to mitigate parasitic lasing in mid-IR fiber amplifiers using a single long period fiber grating is shown. Using tightly confined ultrashort laser pulses at 800 nm, a grating was directly inscribed into the core of an erbium doped fluoride glass fiber showing a strong attenuation down to ...
Source: Optics Express - Category: Physics Authors: Source Type: research
DNA transformation of parasitic nematodes enables novel approaches to validating predictions from genomic and transcriptomic studies of these important pathogens. Notably, proof of principle for CRISPR/Cas9 mutagenesis has been achieved in Strongyloides spp. allowing identification of molecules essential to the functions of sensory neurons that mediate behaviors comprising host finding, invasion and location of predilection sites by parasitic nematodes. Likewise, CRISPR/Cas9 knockout of the developmental regulatory transcription factor Ss-daf-16 has validated its function in regulating morphogenesis of infective third-stag...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
ConclusionsThe high frequency ofL.braziliensis infection in dogs with ulcers and the similarities between the isolates ofL.braziliensis and cutaneous leishmaniasis in dogs and humans in an endemic area of TL, raise the possibility of an important role of dogs in the transmission chain ofL.braziliensis.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Novel parasites can have wide-ranging impacts, not only on host populations, but also on the resident parasite community. Historically, impacts of novel parasites have been assessed by examining pairwise interactions between parasite species. However, parasite communities are complex networks of interacting species. Here we used multivariate taxonomic and trait-based approaches...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
This week's case is from Blaine Mathison. The following were seen on a trichrome-stained stool specimen. They averaged 5 to 7 micrometers in greatest dimension. A Giardia antigen test was positive.Identification?
Source: Creepy Dreadful Wonderful Parasites - Category: Parasitology Source Type: blogs
ConclusionsThe result showed that sampling region, fish status, fish species and sampling month were the main factors influencing infection rate. Regulations or guidelines are urgent in China for the control of Anisakis spp. infection in marine fish.
Source: Food Control - Category: Food Science Source Type: research
Publication date: Available online 15 July 2019Source: Preventive Veterinary MedicineAuthor(s): Alessia Libera Gazzonis, Sergio Aurelio Zanzani, Luca Villa, Maria Teresa ManfrediAbstractThe zoonotic protozoa Toxoplasma gondii is one of the major abortive pathogens in small ruminants. Nevertheless, data on T. gondii infection in goats during lactation and on the presence of T. gondii in goat milk are lacking. A longitudinal study was planned in a T. gondii naturally infected dairy goat farm with the aim of (i) evaluating the variation of anti-T. gondii antibodies in blood and milk during the lactation; (ii) identifying the ...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
J, Domínguez-Bernal G Abstract HisAK70 candidates have successfully been tested in cutaneous (CL) and visceral leishmaniosis (VL) mouse models. Here, we analyse different biomarkers in dog trials after a heterologous immunization strategy with a HisAK70 candidate (plasmid DNA plus adoptive transfer of peripheral blood-derived dendritic cells (DCs) pulsed with the same pathoantigen and CpG ODN as an adjuvant) to explore the antileishmanial activity in an ex vivo canine co-culture system in the presence of Leishmania infantum parasites. In the canine model, the heterologous HisAK70 vaccine could decrease the...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
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