Structure-activity relationship between Zn(2+)-chelated alkylated poly(1-vinylimidazole) and gene transfection.

Structure-activity relationship between Zn(2+)-chelated alkylated poly(1-vinylimidazole) and gene transfection. J Inorg Biochem. 2017 May 10;173:120-125 Authors: Asayama S, Sakata M, Kawakami H Abstract The structure-activity relationship between Zn(2+)-chelated alkylated poly(1-vinylimidazole) (PVIm) and gene transfection has been demonstrated. From a chemical structure perspective, ethylated PVIm (PVIm-Et) chelated the most Zn(2+) ions compared to methylated PVIm (PVIm-Me) and butylated PVIm (PVIm-Bu). The resulting Zn(2+)-chelated PVIm-Et formed more stable complexes with plasmid DNA complex than non-chelated PVIm-Et. From a biological activity perspective, the Zn(2+)-chelated PVIm-Et delivered the highest amount of Zn(2+) ions inside the cell, corresponding to the highest gene transfection. These results suggest that PVIm-Et is the optimal sequence for the chelation of Zn(2+) ions to enhance the gene transfection activity. The structure-activity relationship in this study is expected to offer a unique molecular design for drug/gene delivery systems. PMID: 28511062 [PubMed - as supplied by publisher]
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research