Organic-inorganic hybrid nanoparticles controlled delivery system for anticancer drugs
In this study, iron oxide nanoparticles were prepared then surface modified via diazonium chemistry and coated with chitosan, and its derivative chitosan-grafted polylactic acid. The purpose was to increase the stability of the nanoparticles in physiological solution, heighten drug-loading capacity, prolong the release, reduce the initial burst effect and improve in vitro cytotoxicity of the model drug doxorubicin. The materials were characterized by DLS, ζ-potential, SEM, TGA, magnetization curves and release kinetics studies. Results confirmed the spherical shape, the presence of the coat and the advantages of using chitosan, particularly its amphiphilic derivative, as a coating agent, thereby surpassing the qualities of simple iron oxide nanoparticles. The coated nanoparticles exhibited great stability and high encapsulation efficiency for doxorubicin, at over 500μg per mg of carrier. Moreover, the intensity of the initial burst was clearly diminished after coating, hence represents an advantage of using the hybrid system over simple iron oxide nanoparticles. Cytotoxicity studies demonstrate the increase in cytotoxicity of doxorubicin when loaded in nanoparticles, indirectly proving the role played by the carrier and its surface properties in cell uptake. Graphical abstract
ConclusionsSpinach irrigated with RHW did not significantly affect the populations of fecal indicator bacteria when compared to CW‐irrigated spinach. Repeat irrigation with RCW is not recommended due to increased contamination of E. coli on spinach leaves. Significance and Impact of the StudyRHW may potentially be used as alternative irrigation water without deleteriously affecting the microbiological safety of the spinach.This article is protected by copyright. All rights reserved.
This study suggests that the optimized medium is not only effective but also economical for HSAF production. This article is protected by copyright. All rights reserved.