Analysis of an immunoadjuvant saponin fraction from Quillaja brasiliensis leaves by electrospray ionization ion trap multiple-stage mass spectrometry

Publication date: June 2017 Source:Phytochemistry Letters, Volume 20 Author(s): Federico Wallace, Zohra Bennadji, Fernando Ferreira, Cristina Olivaro An immunoadjuvant saponin fraction from Quillaja brasiliensis leaves was investigated by direct infusion and liquid chromatography/electrospray ionization ion trap multiple-stage mass spectrometry in negative ion mode (DI-ESI-IT-MSn and LC-ESI-IT-MSn). The aglycone and the sequence of the oligosaccharide residues at C-3 and C-28 were characterized based on MS2 and MS3 experiments of the [MH]− ions. According to their [MH]− ions, characteristic product ions and retention times, 27 bidesmosidic saponins, bearing four types of triterpenic aglycones, were tentatively identified. Graphical abstract

Source: Phytochemistry Letters - May 15, 2017 Category: Chemistry Source Type: research