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Dipicolinic acid as a novel spore-inspired excipient for antibody formulation

Publication date: 30 June 2017 Source:International Journal of Pharmaceutics, Volume 526, Issues 1–2 Author(s): Iris L. Batalha, Peng Ke, Esther Tejeda-Montes, Shahid Uddin, Christopher F. van der Walle, Graham Christie Ionic excipients are commonly used in aqueous therapeutic monoclonal antibody (mAb) formulations. Novel excipients are of industrial interest, with a recent focus on Arg salt forms and their application as viscosity reducing and stabilizing additives. Here, we report that the calcium salt of dipicolinic acid (DPA, pyridine-2,6-dicarboxylic acid), uniquely present in nature in the core of certain bacterial spores, reduces the viscosity of a mAb formulated at 150mg/mL, below that achieved by Arg hydrochloride at the same concentration (10mM). DPA also reduced the reversible phase separation of the same formulation, which characteristically occurs for this mAb upon cooling to 4°C. Differential scanning calorimetry and differential scanning fluorimetry did not reveal a conformation destabilisation of the mAb in the presence of 10mM DPA, or by the related quinolinic acid (QA, pyridine-2,3-dicarboxylic acid). However, fluorescence spectrophotometry did reveal localised (aromatic) conformational changes to the mAb attributed to DPA, dependent on the salt form. While precise mechanisms of action remain to be identified, our preliminary data suggest that these DPA salts are worthy of further investigation as novel ionic excipient for biologics formulation. G...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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The most intriguing abandoned settlements, from ancient ruins to modern ghost townsLast week laser scanning revealedthe true scale of the ancient city of Angamuco in western Mexico. The city, built around AD900, is thought to have had 100,000 residents and included pyramids, road systems, vegetable gardens and ball courts. It was a major centre for the Pur épecha people, rivals to the Aztecs. Both cultures collapsed in the 16th century when Europeans introduced typhoid-like diseases to which they had no immunity.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Archaeology Technology Science Source Type: news
Publication date: Available online 24 February 2018 Source:Thermochimica Acta Author(s): Fiona C. Binks, Gabriel Cavalli, Michael Henningsen, Brendan J. Howlin, Ian Hamerton An epoxy resin based on the difunctional diglycidyl ether of bisphenol A (DGEBA) is reacted thermally in the presence of a series of 1,3-dialkylimidazolium based ionic liquids comprising the common cation (1-ethyl-3-methylimidazolium) and an anion (acetate, dicyanamide, or thiocyanate). Dynamic differential scanning calorimetry was used to analyse the formulations comprising DGEBA and the ionic liquid where it was revealed that the lowest and highest ...
Source: Thermochimica Acta - Category: Chemistry Source Type: research
Publication date: 3 May 2018 Source:Composites Science and Technology, Volume 159 Author(s): Zibiao Li, Joseph K. Muiruri, Warintorn Thitsartarn, Xing Zhang, Beng Hoon Tan, Chaobin He In the effort to overcome the shortcomings such as brittleness and poor mechanical stability, and increase the competitive edge of renewable poly(lactic acid) PLA over conventional petroleum-based thermoplastics, silica rubber core-shell nanoparticles for effective PLA toughening were successfully synthesized by sequential ring opening polymerization (ROP). The core-shell structure was designed with silica as inner core, P(CL-mLA) as ‘...
Source: Composites Science and Technology - Category: Science Source Type: research
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Source: Letters in Applied Microbiology - Category: Microbiology Authors: Tags: Original Article Source Type: research
Publication date: 1 May 2018 Source:Colloids and Surfaces B: Biointerfaces, Volume 165 Author(s): Hossam E. Emam, Osama M. Darwesh, Reda M. Abdelhameed Bio-active synthetic fabrics based on polyester (PET) and Nylon were manufactured by in-situ formation of Cu-BTC metal organic framework (MOF). In-growth of Cu-BTC within fabrics was accomplished in one pot simple process. The scanning microscope, X-ray diffraction and infrared spectra were all confirmed the formation of Cu-BTC within fabrics structure and reflected the role of fabrics' building unit in the Cu-BTC preparation. The estimated contents of materials onto fabri...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Conclusion(s) This TMS/fMRI study evaluated a control condition that preserved many of the sensory features of TMS while reducing magnetic field entry. These findings support a relationship between single pulses of TMS and activity in anatomically connected regions, but also underscore the importance of using a sham condition in future TMS/fMRI studies.
Source: Brain Stimulation - Category: Neurology Source Type: research
Publication date: 1 April 2018 Source:Journal of Membrane Science, Volume 551 Author(s): Krishnasri V. Kurada, Tanmay, Sirshendu De Industrial oily water containing oil, grease and dust has been treated using stage-wise filtration, first by sand bed followed by cross-flow ultrafiltration hollow fiber membrane. The sand bed removes majority of suspended particles and reduces the turbidity from 13.6 NTU to 2.6 NTU. Effects of transmembrane pressure drop and cross flow rate on ultrafiltration performance were investigated. A transport phenomena based model under the framework of boundary layer theory was developed to quantif...
Source: Journal of Membrane Science - Category: Materials Science Source Type: research
Authors: Anner P, Mayerhöfer M, Wadsak W, Geleff S, Dudczak R, Haug A, Hacker M, Karanikas G Abstract The current study aimed to determine the optimum diagnostic imaging technique out of magnetic resonance imaging (MRI), 18F-fludeoxyglucose positron emission tomography/computed tomography ([18F]FDG-PET/CT, otherwise known as PET/CT) and [18F]FDG-PET/MRI (otherwise known as PET/MRI) for the pelvic lymph node staging (N-staging) of untreated cervical carcinoma (CC). A total of 27 patients were included in the present study. All patients had undergone pre-treatment with PET/CT and MRI ≤45 days prior to undergo...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Abstract IntroductionTwin vaginal delivery presents a unique clinical challenge for obstetricians. The twin birth study demonstrated the safety of planned vaginal delivery regarding neonatal outcomes. However, that study lacked description of the risk factors associated with and the outcome of unplanned cesarean section. The aim of this study is to identify potential risk factors for cesarean section and delivery related neonatal morbidity and mortality in women with twin pregnancy attempting vaginal delivery. Material and methodsA retrospective cohort study including 1070 women with twin pregnancy that underwent a trial o...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: Original Research Article Source Type: research
(NEW YORK) — Thirteen years after building a state-of-the-art lab for the world’s most dangerous germs, the nation’s top public health agency is asking for more than $400 million to build a new one. Officials at the Centers for Disease Control and Prevention say the current lab building in Atlanta is quickly wearing down, and cannot be upgraded without shutting down the facility for years. The lab investigates deadly and exotic germs like Ebola, smallpox and dangerous new forms of flu.The agency disclosed its plans for a new lab on Friday. The CDC lab is one of only eight U.S. labs with the security and s...
Source: TIME: Science - Category: Science Authors: Tags: Uncategorized APH healthytime medicine onetime Source Type: news
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