Dipicolinic acid as a novel spore-inspired excipient for antibody formulation

Publication date: 30 June 2017 Source:International Journal of Pharmaceutics, Volume 526, Issues 1–2 Author(s): Iris L. Batalha, Peng Ke, Esther Tejeda-Montes, Shahid Uddin, Christopher F. van der Walle, Graham Christie Ionic excipients are commonly used in aqueous therapeutic monoclonal antibody (mAb) formulations. Novel excipients are of industrial interest, with a recent focus on Arg salt forms and their application as viscosity reducing and stabilizing additives. Here, we report that the calcium salt of dipicolinic acid (DPA, pyridine-2,6-dicarboxylic acid), uniquely present in nature in the core of certain bacterial spores, reduces the viscosity of a mAb formulated at 150mg/mL, below that achieved by Arg hydrochloride at the same concentration (10mM). DPA also reduced the reversible phase separation of the same formulation, which characteristically occurs for this mAb upon cooling to 4°C. Differential scanning calorimetry and differential scanning fluorimetry did not reveal a conformation destabilisation of the mAb in the presence of 10mM DPA, or by the related quinolinic acid (QA, pyridine-2,3-dicarboxylic acid). However, fluorescence spectrophotometry did reveal localised (aromatic) conformational changes to the mAb attributed to DPA, dependent on the salt form. While precise mechanisms of action remain to be identified, our preliminary data suggest that these DPA salts are worthy of further investigation as novel ionic excipient for biologics formulation. G...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Conclusion. The multistep, patient-specific SGTS is useful for intraoperative pedicle screw (PS) navigation in the cervical and thoracic spine. This method improves the accuracy of PS insertion and reduces the operating time and radiation exposure during spinal fixation surgery. Level of Evidence: 3
Source: Spine - Category: Orthopaedics Tags: SURGERY Source Type: research
Conclusion. The spine multiple WSM is an extremely rare condition, which has a malignant behavior and it seems that it is spread by cerebrospinal fluid seeding. Surgical removal is hard and requires experience and appropriate equipment. Level of Evidence: 4
Source: Spine - Category: Orthopaedics Tags: CASE REPORT Source Type: research
Conclusion. Our case depicts a model of slow creeping deformation due to left C1 superior articular facet dysplasia. An abnormal facet slope allowed the occipital condyle to migrate posteriorly and medially leading to crowding of the left C1 foramen. Although the etiology was congenital, the neck spasm was delayed till fourth decade. A secondary C1 foramen stenosis led to C1 occipital neuralgia that presented as an intractable headache. Level of Evidence: 5
Source: Spine - Category: Orthopaedics Tags: CASE REPORT Source Type: research
Authors: Fossi F, Robba C, Rota M, Vargiolu A, Lagravinese D, Volpi P, Citerio G Abstract BACKGROUND: Evidence-based indications for intracranial pressure (ICP) monitoring in patients with traumatic brain injury (TBI) are lacking. The aim of this study was to analyze the main factors that guided the decision-making of invasive ICP monitoring in a large cohort of TBI patients from our institution. METHODS: This is a retrospective, single centre, observational study including adult TBI patients consecutively admitted to our NeuroIntensive Care Unit over 20 years. Logistic regression analyses were performed to ide...
Source: Journal of Neurosurgical Sciences - Category: Neurosurgery Tags: J Neurosurg Sci Source Type: research
We describe a 32-year-old woman with spontaneous regression of HL and review the literature. The patient presented with cervical lymphadenopathy and was diagnosed with stage IIA classical HL. The patient refused to receive any treatment for her disease. Positron emission tomography/computed tomography carried out 2 years later showed complete regression of the lymphadenopathy, without pathological uptake of fluorodeoxyglucose. At the last follow-up, 3.5 years after the initial presentation, the patient is with no evidence of disease. During workup for the HL, concomitant papillary thyroid carcinoma was diagnosed, for which...
Source: Acta Haematologica - Category: Hematology Source Type: research
Authors: Rubtsova NY, Heckmann RA, Smit WJ, Luus-Powell WJ, Halajian A, Roux F Abstract The present study was performed to observe histopathological effects of Oculotrema hippopotami Stunkard, 1924 infection in the eye of Hippopotamus amphibius, as well as to reveal new details of morphology and structural features of this monogenean and its comparison between 2 age stages of the parasite. This was done using both light and scanning electron microscopy, energy dispersive X-ray analysis (EDXA) and histopathology. The presence of a mixture of different generations (adult and sub-adult) in one host individual is commo...
Source: Korean Journal of Parasitology - Category: Parasitology Tags: Korean J Parasitol Source Type: research
Publication date: Available online 14 November 2018Source: Saudi Pharmaceutical JournalAuthor(s): Sultan M. Alshehri, Faiyaz Shakeel, Mohamed A. Ibrahim, Ehab M. Elzayat, Mohammad Altamimi, Kazi Mohsin, Osaid T. Almeanazel, Musaed Alkholief, Abdullah Alshetaili, Bader Alsulays, Fars K. Alanazi, Ibrahim A. AlsarraAbstractApigenin (APG) is a poorly soluble bioactive compound/nutraceutical which shows poor bioavailability upon oral administration. Hence, the objective of this research work was to develop APG solid dispersions (SDs) using different techniques with the expectation to obtain improvement in its in vitro dissoluti...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
The Putian network of 8,000 facilities, which expanded rapidly with little government oversight, is facing national outrage and scrutiny over a death and fraud allegations.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Hospitals China Rural Areas National Health and Family Planning Commission (China) Baidu Inc Chen Deliang Source Type: news
No matter how long I deal with periodic blood tests, abdominal CTs and mammograms, they always trigger a huge wave of ‘ scanxiety. ’
Source: NYT Health - Category: Consumer Health News Authors: Tags: Ovarian Cancer Mammography Mastectomy Source Type: news
Morteza Esmaeili, Nassim Tayari, Tom Scheenen, Mattijs Elschot, Elise Sandsmark, Helena Bertilsson, Arend Heerschap, Kirsten M. Seln æs, Tone F. Bathen
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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