Dipicolinic acid as a novel spore-inspired excipient for antibody formulation

Publication date: 30 June 2017 Source:International Journal of Pharmaceutics, Volume 526, Issues 1–2 Author(s): Iris L. Batalha, Peng Ke, Esther Tejeda-Montes, Shahid Uddin, Christopher F. van der Walle, Graham Christie Ionic excipients are commonly used in aqueous therapeutic monoclonal antibody (mAb) formulations. Novel excipients are of industrial interest, with a recent focus on Arg salt forms and their application as viscosity reducing and stabilizing additives. Here, we report that the calcium salt of dipicolinic acid (DPA, pyridine-2,6-dicarboxylic acid), uniquely present in nature in the core of certain bacterial spores, reduces the viscosity of a mAb formulated at 150mg/mL, below that achieved by Arg hydrochloride at the same concentration (10mM). DPA also reduced the reversible phase separation of the same formulation, which characteristically occurs for this mAb upon cooling to 4°C. Differential scanning calorimetry and differential scanning fluorimetry did not reveal a conformation destabilisation of the mAb in the presence of 10mM DPA, or by the related quinolinic acid (QA, pyridine-2,3-dicarboxylic acid). However, fluorescence spectrophotometry did reveal localised (aromatic) conformational changes to the mAb attributed to DPA, dependent on the salt form. While precise mechanisms of action remain to be identified, our preliminary data suggest that these DPA salts are worthy of further investigation as novel ionic excipient for biologics formulation. G...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 18 July 2018Source: Journal of Herbal MedicineAuthor(s): Eliana Harue Endo, Gustavo Meireles Costa, Regina Yasuko Makimori, Tania Ueda-Nakamura, Celso Vataru Nakamura, Benedito Prado Dias FilhoAbstractThe present study investigated the anti-staphylococcal effect of extracts of three species of plants (Rosmarinus officinalis, Punica granatum, Tetradenia riparia) against planktonic and biofilm cells of Staphylococcus aureus, including MRSA and MSSA. Minimal inhibitory and bactericidal concentrations (MIC and MBC) of hydroalcoholic extracts prepared with the leaves of R. officinalis and T. r...
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Source: Materials Science in Semiconductor Processing - Category: Materials Science Source Type: research
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Source: Materials Science in Semiconductor Processing - Category: Materials Science Source Type: research
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Source: Journal of Physics and Chemistry of Solids - Category: Physics Source Type: research
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Source: Health News - UPI.com - Category: Consumer Health News Source Type: news
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