Procaine Inhibits Proliferation and Migration of Colon Cancer Cells Through Inactivation of the ERK/MAPK/FAK Pathways By Regulation of RhoA.

Procaine Inhibits Proliferation and Migration of Colon Cancer Cells Through Inactivation of the ERK/MAPK/FAK Pathways By Regulation of RhoA. Oncol Res. 2017 May 11;: Authors: Li C, Gao S, Li X, Li C, Ma L Abstract Colon cancer is one of the lethal varieties of cancer. Chemotherapy remains to be one of the principal treatment approaches for colon cancer. Anticancer activity of procaine (PCA), which is a local anesthetic drug, has been explored in different studies. In our study, we aimed to explore the anticancer effect of PCA on colon cancer and its underlying mechanism. The results showed that PCA significantly inhibited cell viability, increased the percentage of apoptotic cells and decreased the expression level of RhoA in HCT116 cells in a dose dependent manner (P<0.05 or P<0.01). Moreover, PCA increased the proportion of HCT116 cells in G1 phase as well as down-regulated Cyclin D1 and Cyclin E expressions (P<0.05). In addition, we also found that PCA remarkably inhibited cell migration in HCT116 cells (P<0.01). However, all these effects of PCA on cell proliferation, apoptosis and migration were significantly reversed by PCA + pc-RhoA (P<0.05 or P<0.01). Besides, PCA significantly decreased the levels of p-ERK, p-p38MAPK and p-FAK, but PCA + pc-RhoA rescued these effects. Furthermore, ERK inhibitor (PD098059), p38MAPK inhibitor (SB203580) and FAK inhibitor (Y15) reversed these results. These data indicated that...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research