Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme

Publication date: 30 June 2017 Source:International Journal of Pharmaceutics, Volume 526, Issues 1–2 Author(s): Márcia Alves, Nicole S.M. Vieira, Luís Paulo N. Rebelo, João M.M. Araújo, Ana B. Pereiro, Margarida Archer Since the approval of recombinant human insulin by FDA in 1982, more than 200 proteins are currently available for pharmaceutical use to treat a wide range of diseases. However, innovation is still required to develop effective approaches for drug delivery. Our aim is to investigate the potential use of fluorinated ionic liquids (FILs) as drug delivery systems (DDS) for therapeutic proteins. Some initial parameters need to be assessed before further studies can proceed. This work evaluates the impact of FILs on the stability, function, structure and aggregation state of lysozyme. Different techniques were used for this purpose, which included differential scanning fluorimetry (DSF), spectrophotometric assays, circular dichroism (CD), dynamic light scattering (DLS), and scanning and transmission electron microscopy (SEM/TEM). Ionic liquids composed of cholinium-, imidazolium- or pyridinium- derivatives were combined with different anions and analysed at different concentrations in aqueous solutions (below and above the critical aggregation concentration, CAC). The results herein presented show that the addition of ionic liquids had no significant effect on the stability and hydrolytic activity of lysozyme. Moreover, a distinct ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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The objective of this study was to compare the accuracy of ultrasonographyversus external palpation in localizing the cricothyroid membrane.Methods In total, 223 subjects with abnormal neck anatomy who were scheduled for neck computed-tomography scan at University Health Network hospitals in Toronto, Canada, were randomized into two groups: external palpation and ultrasound. The localization points of the cricothyroid membrane determined by ultrasonography or external palpation were compared to the reference midpoint (computed-tomography point) of the cricothyroid membrane by a radiologist who was blinded to group allocati...
Source: Anesthesiology - Category: Anesthesiology Source Type: research
ConclusionDaily vibration treatment produced clinically significant shortening of the time needed for mandibular incisor anterior-posterior correction with clear aligners.
Source: Dental Technology Blog - Category: Dentistry Source Type: news
Conclusions: Endothoracic paragangliomas, rare and often asymptomatic tumors, are of difficult diagnosis and should be considered malignant tumors, due to the potential aggressive behaviour of cases with high mitotic index and the frequent possibility of recurrence and metastases. Surgical resection is the treatment of choice and careful intraoperative manipulation is recommended, due to the high vascularity of these tumors, to prevent complications. After complete excision, long-term prognosis is generally good. However, even after surgical removal, a close, periodical and life-long follow-up is mandatory. PMID: 30416779 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Conclusions: The continuous suture dividing and equal suture tightening method is convenient, feasible, and safe for bronchial anastomosis in VATS sleeve lobectomy. It can effectively avoid the entanglement of the suture threads, thereby enabling the widespread adoption of VATS sleeve lobectomy. PMID: 30416767 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Credit: University of Nottingham ___ Will it be about a new generation of portable brain scanners that can significantly lower the price of research, like the one being developed by researchers at the University of Nottingham and University College London in the UK? Or will it be about sophisticated neural interfaces, like the ones the US military is working on, to change the ways our minds interact with the world around us? Or, perhaps, will it be about inexpensive, data-rich approaches to detect patterns in your smartphone usage and detect mental health and brain disorders in their early stages &mdas...
Source: SharpBrains - Category: Neuroscience Authors: Tags: Cognitive Neuroscience Health & Wellness Technology brain disorders brain scanners Mental-Health neural interfaces Neurotechnology smartphone usage Source Type: blogs
CONCLUSIONS: Despite limitations, study results support the view that patients after non-severe TBI with ASDH and with lateral ventricle asymmetry, defined as LVR> 1.48, require surgical treatment. LVR seems to be indirect, but still the closest method to quantify intracranial compliance. Thus, in the selected group of patients without clinical symptoms of critically diminished compensatory reserve, LVR could indicate those who need a surgical decompression. PMID: 30417687 [PubMed - as supplied by publisher]
Source: Brain Injury - Category: Neurology Tags: Brain Inj Source Type: research
CONSTRUCTION OF SPATIOTEMPORAL INFANT CORTICAL SURFACE ATLAS OF RHESUS MACAQUE. Proc IEEE Int Symp Biomed Imaging. 2018 Apr;2018:704-707 Authors: Wang F, Lian C, Xia J, Wu Z, Duan D, Wang L, Shen D, Li G Abstract As a widely used animal model in MR imaging studies, rhesus macaque helps to better understand both normal and abnormal neural development in the human brain. However, the available adult macaque brain atlases are not well suitable for study of brain development at the early postnatal stage, since this stage undergoes dramatic changes in brain appearances and structures. Building age matched a...
Source: Proceedings - International Symposium on Biomedical Imaging - Category: Radiology Tags: Proc IEEE Int Symp Biomed Imaging Source Type: research
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Source: Magnetic Resonance in Medical Sciences - Category: Radiology Tags: Magn Reson Med Sci Source Type: research
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Source: Magnetic Resonance in Medical Sciences - Category: Radiology Tags: Magn Reson Med Sci Source Type: research
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Source: Molecular Imaging and Biology - Category: Molecular Biology Source Type: research
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