Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme
Publication date: 30 June 2017 Source:International Journal of Pharmaceutics, Volume 526, Issues 1–2 Author(s): Márcia Alves, Nicole S.M. Vieira, Luís Paulo N. Rebelo, João M.M. Araújo, Ana B. Pereiro, Margarida Archer Since the approval of recombinant human insulin by FDA in 1982, more than 200 proteins are currently available for pharmaceutical use to treat a wide range of diseases. However, innovation is still required to develop effective approaches for drug delivery. Our aim is to investigate the potential use of fluorinated ionic liquids (FILs) as drug delivery systems (DDS) for therapeutic proteins. Some initial parameters need to be assessed before further studies can proceed. This work evaluates the impact of FILs on the stability, function, structure and aggregation state of lysozyme. Different techniques were used for this purpose, which included differential scanning fluorimetry (DSF), spectrophotometric assays, circular dichroism (CD), dynamic light scattering (DLS), and scanning and transmission electron microscopy (SEM/TEM). Ionic liquids composed of cholinium-, imidazolium- or pyridinium- derivatives were combined with different anions and analysed at different concentrations in aqueous solutions (below and above the critical aggregation concentration, CAC). The results herein presented show that the addition of ionic liquids had no significant effect on the stability and hydrolytic activity of lysozyme. Moreover, a distinct ...
CONCLUSION: The results indicate that 4 per cent boric acid solution and vinegar with water (1:1) are potent inhibitors of biofilms from methicillin-resistant S aureus and quinolone-resistant P aeruginosa, and provide safe pH levels for avoiding ototoxicity. PMID: 29909794 [PubMed - as supplied by publisher]
CONCLUSION: Existing evidence supports the incorporation of this inexpensive instrument in the armamentarium for resecting lateral extension of juvenile nasopharyngeal angiofibroma during a transpalatal approach. PMID: 29909791 [PubMed - as supplied by publisher]
CONCLUSION: Pre-operative combined magnetic resonance imaging and computed tomography helped predict disease in the frontal sinus better than computed tomography alone. A close working relationship between the ENT and radiology departments is important for accurate tumour localisation. PMID: 29909780 [PubMed - as supplied by publisher]
We report on the results of a 6-year French tricenter study on 1,124 cases of fetal USDA for whom a comprehensive molecular study was performed for CF.Results: Among the 37 CF fetuses, 5 (13.5%) presented with isolated NVFGB at ultrasound (US) examination at 24 –31 weeks of gestation. This sign was more frequently observed in CF fetuses than in non-CF fetuses, with a likelihood ratio of 2.7. The genotypes included three c.1521_1523del (F508del) homozygous cases and two compound heterozygous cases for a frequent and a rare CF-causing variant.Discussion: These observations highlight the importance to report on the pres...
In this study, ZIF-8 modified g-C3N4 (ZIF-8/g-C3N4) composites were successfully synthesized by hydrothermal reaction. The properties of the ZIF-8/g-C3N4 composites were analyzed by X-ray diffraction (XRD), scanning electron microscope (SEM),... The content of this RSS Feed (c) The Royal Society of Chemistry
[Nation] The controversial acquisition of the Sh8 billion CT scan machines has come back to haunt the government after a parliamentary committee summoned Health Principal Secretary Peter Tum to explain why the cost of the machines was inflated.
I have blogged in the past about provider-to-provider teleconsults (see:The Need for More Research in Provider-to-Provider Telemedicine). This type of telemedicine is often overlooked or underreported. A recent article addressed how video consults have improved survival rates for liver disease patients (see:Video consults boost survival rates for liver disease patients by 54 percent). Below is an excerpt from the article:Patients of primary care physicians who participated in video consultations with liver disease specialists had a 54 percent higher survival rate than patients whose PCPs didn ’t leverage th...
Conclusion: This study describes the first MRI-compatible hydrodynamic simulator of CSF motion in the cervical spine with anatomically realistic NR. NR were found to impact CSF velocity profiles to a great degree. Significance: CSF hydrodynamics are thought to be altered in craniospinal disorders such as Chiari I malformation. MRI scanning techniques and protocols can be used to quantify CSF flow alterations in disease states. The provided in vitro models can be used to test the reliability of these protocols across MRI scanner manufacturers and machines.
Conclusion and significance: The proposed set of quantitative methods supports the development and troubleshooting of electrically-active mechatronic devices for use in sensorimotor protocols with fMRI, and may be used for future testing of such devices.
Nurses at the first hospice in the UK to trial a ground-breaking scanner designed to detect pressure damage say the device has helped them act sooner to stop ulcers developing and raises questions about the value of traditional skin assessments.