Combining new tools to assess renal function and morphology: a holistic approach to study the effects of aging and a congenital nephron deficit.

Combining new tools to assess renal function and morphology: a holistic approach to study the effects of aging and a congenital nephron deficit. Am J Physiol Renal Physiol. 2017 May 10;:ajprenal.00329.2015 Authors: Geraci S, Chacón-Caldera J, Cullen-McEwen LA, Schad LR, Sticht C, Puelles VG, Bertram JF, Gretz N Abstract Recently, new methods for assessing renal function in conscious mice (transcutaneous assessment) and for counting and sizing all glomeruli in whole kidneys (MRI) were described. In the present study, these methods were used to assess renal structure and function in aging mice, and in mice born with a congenital low nephron endowment. Age-related nephron loss was analysed in adult C57BL/6 mice (10-50 weeks of age) and congenital nephron deficit was assessed in glial cell line-derived neurotrophic factor heterozygous (GDNF HET) null mutant mice. Renal function was measured through the transcutaneous quantitation of FITC-sinistrin half-life (t1/2) in conscious mice. MRI was used to image, count and size cationic-ferritin labelled glomeruli in whole kidneys ex vivo. Design-based stereology was used to validate the MRI measurements of glomerular number and mean volume. In adult C57BL/6 mice, older age was associated with fewer and larger glomeruli, and a rightwards shift in the glomerular size distribution. These changes coincided with a decrease in renal function. GNDF HET mice had a congenital nephron deficit that was a...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research