Oxidative imbalance and kidney damage in spontaneously hypertensive rats: activation of extrinsic apoptotic pathway

We examined plasmatic pro-oxidant and antioxidant status showing a significant alteration in oxidative balance in SHR. As index of oxidative damage, we evaluated lipid peroxidation in kidney, liver and skeletal muscle, detecting a significant rise in lipid peroxidation levels in all SHR tissues, particularly relevant in kidney. In addition, we analyzed the expression of cytoplasmic antioxidant enzymes, SOD1 and GSTP1. In SHR liver, SOD1 expression slight increased while we have not detected any variation in other tissues. Concerning GSTP1, SHR renal tissues did not display variations in enzyme expression, while, in the other tissues, we observed a significant increase of both monomeric and pro-apoptotic dimeric form of the enzyme. By analyzing apoptotic signal, we founded JNK activation in all SHR tissues, but only kidney presented extrinsic apoptotic pathway activation. Our results suggest that, in hypertensive animals with preserved renal function, despite the remarkable oxidative damage of renal tissues, only the extrinsic apoptotic pathway is activated.

Source: Clinical Science - May 11, 2017 Category: Biomedical Science Authors: La Russa, D., Brunelli, E., Pellegrino, D. Tags: PublishAheadOfPrint Source Type: research