The role of iNKT cells on the phenotypes of allergic airways in a mouse model

Publication date: Available online 5 May 2017 Source:Pulmonary Pharmacology & Therapeutics Author(s): Lennart K.A. Lundblad, Nazey Gülec, Matthew E. Poynter, Victoria L. DeVault, Oliver Dienz, Jonathan E. Boyson, Nirav Daphtary, Minara Aliyeva, Jennifer L. Ather, Felix Scheuplein, Robert Schaub iNKT cells and mast cells have both been implicated in the syndrome of allergic asthma through their activation-induced release of Th2 type cytokines and secretion of histamine and other mediators, respectively, which can promote airways hyperresponsiveness (AHR) to agents such as methacholine. However, a mechanistic link between iNKT cells and mast cell recruitment or activation has never been explored. Our objective was to determine whether iNKT cells are necessary for the recruitment of mast cells and if iNKT cells can influence the acute allergen induced bronchoconstriction (AIB) caused by mast cell mediator release. To do so, we pharmacologically eliminated iNKT cells using a specific antibody (NKT-14) and examined its impact on airway inflammation and physiological phenotype. In mice treated with NKT-14, the elimination of iNKT cells was sufficient to prevent AHR and pulmonary eosinophilic inflammation elicited by administration of the iNKT cell agonist, αGalCer. In mice treated with NKT-14 and then sensitized and challenged with house dust mite extract (HDM), eliminating the iNKT cells significantly reduced both AHR and AIB but did not affect pulmonary inf...
Source: Pulmonary Pharmacology and Therapeutics - Category: Respiratory Medicine Source Type: research