Culturing Stem Cells in 3-D

Requires Potent Media + SupplementsNeuromics is responding to the many challenges our clients face in building3-D, in-vivo like, cell- based assays. We do this by offering the most potentMedia plusSupplements likeFGFS.Here ' s a protocol for single cell 3-D assays using hMSCs and Hydrogels. It features use of ourISOKineTM FGFImages: Cell-centering in cytocompatible microgels enables long-term single-cell 3D culture by preventing cell escape. a) Qualification of Dex-TA microgel crosslinking as a function of the microemulsion flow rate (Qemulsion) and concentration of the H2O2 feed ([H2O2]feed). Blue, green, and red indicate incomplete crosslinking, complete crosslinking, and H2O2 excess, respectively. b,c) Amplex Red assay to quantify the concentration of residual H2O2 ([H2O2]emulsion) in Dex-TA microgel precursor droplets and crosslinked microgels after their retrieval from the diffusion-based crosslinking platform. d) The microencapsulation procedure had no detrimental effect on short-term cell survival. e) Delayed crosslinking resulted in 4 ± 1% cell escape after 7 d of in vitro culture, as compared to 27 ± 5% cell escape when using coupled emulsification and gelation. f) The number of encapsulated cells per microgel tightly followed the Poisson distribution and remained similar throughout long-term (28 d) of in vitro culture, which confirmed that cell centering prevents cell escape. g–i) MSCs encapsulated in delayed enzymatically crosslinked microgels remaine...
Source: Neuromics - Category: Neuroscience Tags: 3-D Cell based Assays adult stem cells FGF Basic FGF2 Fibroblast Growth Factor 2 Fibroblast Growth Factor Basic hMSCs human Mesenchymal Stem Cell Based Assays ISOKine ISOKineFGF Source Type: news