Fractionation of UV-B absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography

Publication date: Available online 22 March 2017 Source:Phytochemistry Letters Author(s): R. Boulho, J. Le Roux, C. Le Quémener, G. Audo, N. Bourgougnon, G. Bedoux Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310nm. Graphical abstract

Source: Phytochemistry Letters - May 3, 2017 Category: Chemistry Source Type: research