Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4   Å resolution

The X-ray crystal structure of the DNA tetradecamer sequence d(CCGGGGTACCCCGG)2 is reported at 1.4   Å resolution in the tetragonal space group P41212. The sequence was designed to fold as a four-way junction. However, it forms an A-type double helix in the presence of barium chloride. The metal ion could not be identified in the electron-density map. The crystallographic asymmetric unit consists of one A-type double helix with 12 base pairs per turn, in contrast to 11 base pairs per turn for canonical A-DNA. A large number of solvent molecules have been identified in both the grooves of the duplex and around the backbone phosphate groups.

Source: Acta Crystallographica Section F - April 26, 2017 Category: Biochemistry Authors: Karthik, S. Thirugnanasambandam, A. Mandal, P.K. Gautham, N. Tags: d(CCGGGGTACCCCGG)2 A-DNA duplex X-ray crystallography tetragonal space group research communications Source Type: research