Development and applications of two colorimetric and fluorescent indicators for Hg(2+) detection.

Development and applications of two colorimetric and fluorescent indicators for Hg(2+) detection. J Inorg Biochem. 2017 Apr 09;172:23-33 Authors: Long Y, Yang MP, Yang BQ Abstract Two rhodamine-active probes RBAI (Rhodamine B-di-Aminobenzene-phenyl Isothiocyanate) and RGAI (Rhodamine 6G-di-Aminobenzene-phenyl Isothiocyanate) were designed, synthesized and characterized. The probes were developed as fluorescent and colorimetric chemodosimeters in ethanol-water solution with a broad pH span (5-10) and high selectivity toward Hg(2+) but no significant response toward other common competitive cations. The Hg(2+)-promoted ring opening of spirolactam of the rhodamine moiety induced cyclic guanylation of the thiourea moiety, which resulted in the dual chromo- and fluorogenic observation (off-on). Cytotoxicity and bioimaging studies by L929 living cells and living mice indicated that the probes were negligible cytotoxicity, cell permeable and suitable for detecting Hg(2+) in biological environments. Moreover, the new probes not only displayed excellent abilities for the successful detection of Hg(2+) in L929 living cells and living mice but also able to detect Hg(2+) by adsorbing on solid surfaces and quantitative detection of Hg(2+) in real water samples with good recovery (more than 90%), indicating that they have promising prospect for application for Hg(2+) sensing in environmental and biological sciences. PMID: 28419923 [PubMed ...
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research