Human Neuronal Cell Based Assay: A New In Vitro Model For Toxicity Evaluation Of Ciguatoxin

Publication date: Available online 14 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Teresa Coccini, Francesca Caloni, Uliana De Simone Ciguatoxins (CTXs) are emerging marine neurotoxins representing the main cause of ciguatera fish poisoning, an intoxication syndrome which configures a health emergency and constitutes an evolving issue constantly changing due to new vectors and derivatives of CTXs, as well as their presence in new non-endemic areas. The study applied the neuroblastoma cell model of human origin (SH-SY5Y) to evaluate species-specific mechanistic information on CTX toxicity. Metabolic functionality, cell morphology, cytosolic Ca2+ i responses, neuronal cell growth and proliferation were assessed after short- (4–24h) and long-term exposure (10days) to P-CTX-3C. In SH-SY5Y, P-CTX-3C displayed a powerful cytotoxicity requiring the presence of both Veratridine and Ouabain. SH-SY5Y were very sensitive to Ouabain: 10 and 0.25nM appeared the optimal concentrations, for short- and long-term toxicity studies, respectively, to be used in co-incubation with Veratridine (25μM), simulating the physiological and pathological endogenous Ouabain levels in humans. P-CTX-3C cytotoxic effect, on human neurons co-incubated with OV (Ouabain+Veratridine) mix, was expressed starting from 100pM after short- and 25pM after long-term exposure. Notably, P-CTX-3C alone at 25nM induced cytotoxicity after 24h and prolonged exposure. This human brain-derive...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

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We report the case of a 20-year-old male from the department of Amazonas who presented with nine days of disease characterized by multiorgan failure (neurological, renal, hepatic, respiratory, and hematological involvement). He received antibiotic treatment, as well as, transfusion, dialysis, hemodynamic, and ventilatory support. Despite the severity of the clinical condition, he evolved favorably. YF was confirmed by Rt-PCR and positive serology was obtained for leptospira by ELISA and microagglutination. However, from a laboratory point of view, real co-infection by yellow fever and leptospira could not be demonstrated. ...
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
Out of control proliferation of toxic phytoplankton, called harmful algal blooms (HABs), have a significant economic impact on bivalve aquaculture and harvesting in coastal waters. Some phytotoxins, such as paralytic shellfish toxins (PSTs), are of concern...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Home and Consumer Product Safety Source Type: news
Conclusions: Clinical, laboratory and radiological features of histoplasmosis can mimic other conditions, resulting in unnecessary invasive diagnostic procedures. However, a history of travel to endemic areas and of exposure to risk factors (such as visits to caves and presence of bats) should trigger the clinical suspicion of histoplasmosis. Treatment may be indicated in severe or prolonged disease. PMID: 31959091 [PubMed - as supplied by publisher]
Source: Pathogens and Global Health - Category: International Medicine & Public Health Tags: Pathog Glob Health Source Type: research
ConclusionLack of knowledge regarding the causes of death and careless attitude towards the suicidal behavior and, the health and welfare of detainees are avoidable reasons for custody-related deaths.
Source: Journal of Forensic and Legal Medicine - Category: Forensic Medicine Source Type: research
Publication date: Available online 22 January 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Ronel Pienaar, P. Christo Troskie, Antoinette I. Josemans, Fred T. Potgieter, Boitumelo B. Maboko, Abdalla A. Latif, Ben J. MansAbstractThe Theileria are apicomplexan parasites transmitted by ticks to vertebrate hosts. Most Theileria species exhibit some form of host or vector specificity, since under endemic conditions only a limited number of tick species act as vectors and not all vertebrate hosts are able to maintain a persistent carrier state. Data for Theileria sp. (buffalo) suggest host ...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Conditions:   Refractory Cancer;   Refractory Neoplasm;   Cancer Pediatric;   Refractory Tumor;   Pediatric Cancer;   Pediatric Brain Tumor;   Neuroblastoma;   Neuroblastoma Recurrent;   Pediatric Lymphoma;   Pediatric Meningioma Interventions:   Drug: 9-ING-41;   Drug: Irinotecan Sponsors:   Actuate Therapeutics Inc.;   Developmental Therapeutics Consortium Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Conditions:   Neuroblastoma;   Pediatric Solid Tumor Interventions:   Procedure: Tissue collection;   Biological: GVAX vaccine;   Drug: Nivolumab;   Drug: Ipilimumab Sponsor:   Dana-Farber Cancer Institute Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Publication date: Available online 23 January 2020Source: MethodsXAuthor(s): Huira C. Kopera, Roland S. Hilgarth, Tonya L. Kopas, Thomas M. LaniganAbstractAdenovirus-associated virus is a powerful vector system for transducing cells in vivo. It is widely used in animal systems due to high transduction efficiency of non-dividing cells with more than a dozen serotypes that have preferential tissue tropism. The viral genome remains episomal in the nucleus but maintains sustained expression in terminally differentiated cells for several weeks to months. Despite the popularity of recombinant AAV (rAAV) vectors, quality control ...
Source: MethodsX - Category: Science Source Type: research
Source: Clinical Toxicology - Category: Toxicology Authors: Source Type: research
[This Day] With the recent resurgence of Lassa fever across endemic states in the country, the Edo State Government has firmed up mechanisms to fight the menace with the donation of 200 Personal Protective Equipment (PPE) to the Irrua Specialist Teaching Hospital, as part of efforts to curb the spread of the viral disease in the state.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
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