DNA plasmid vaccine carrying chlamydia trachomatis (Ct) major outer membrane and human papillomavirus 16L2 proteins for anti-Ct infection.

DNA plasmid vaccine carrying chlamydia trachomatis (Ct) major outer membrane and human papillomavirus 16L2 proteins for anti-Ct infection. Oncotarget. 2017 Mar 27;: Authors: Wang L, Cai Y, Xiong Y, Du W, Cen D, Zhang C, Song Y, Zhu S, Xue X, Zhang L Abstract Chlamydia trachomatis (Ct) is one of the most frequently encountered sexual infection all over the world, yielding tremendous reproductive problems (e.g. infertility and ectopic pregnancy) in the women. This work described the design of a plasmid vaccine that protect mice from Ct infection, and reduce productive tract damage by generating effective antibody and cytotoxic T cell immunity. The vaccine, s was composed of MOMP multi-epitope and HPV16L2 genes carried in pcDNA plasmid (i.e. pcDNA3.1/MOMP/HPV16L). In transfection, the vaccine expressed the chimeric genes (i.e. MOMP and HPV16L2), as demonstrated via western blot, RT-PCR and fluorescence imaging. In vitro, the vaccine transfected COS-7 cells and expressed the proteins corresponding to the genes carried in the vaccine. Through intramuscular immunization in BALB/c mice, the vaccine induced higher levels of anti-Ct IgG titer, anti-HPV16L2 IgG titer in serum and IgA titer in local mucosal secretions, compared to plasmid vaccines that carry only Ct MOMP multi-epitope or HPV16L2 chimeric component only. In mice intravaginally challenged with Ct, the vaccines pcDNA3.1/MOMP/HPV16L2 generated a higher level of genital protection compared to other vacci...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research

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Food allergy prevalence has increased over the past 2  decades and is estimated to affect 8% of children and 4% to 10% of adults. There is an unmet need to evaluate new therapeutic modalities that may decrease the risk of food-induced anaphylaxis and improve patients’ quality of life. Oral, epicutaneous, and sublingual food immunotherapies have diff erent safety and efficacy profiles, and their long-term outcome and applicability are unclear. Food allergy trials are currently evaluating different biologics (given as monotherapy or adjunct to immunotherapy), modified food proteins, DNA vaccines, and fecal microbiota transplantation.
Source: Immunology and Allergy Clinics of North America - Category: Allergy & Immunology Authors: Source Type: research
Monitoring fetal heart rate during pregnancy is essential to assist clinicians in making more timely decisions. Non-invasive monitoring of fetal heart activities using abdominal ECGs is useful for diagnosis of heart defects. However, the extracted fetal ECGs are usually too weak to be robustly detected. Thus, it is a necessity to enhance fetal R-peak since their peaks may be hidden within the signal due to the immaturity of the fetal cardiovascular system. Therefore, to improve the detection of the fetal heartbeat, a novel fetal R-peak enhancement technique was proposed to statistically generate the weighting mask accordin...
Source: IEEE Transactions on Biomedical Engineering - Category: Biomedical Engineering Source Type: research
Purpose To identify and examine the correlates of multiple bacterial sexually transmitted infection (STI) hot spot counties in the United States. Methods We assembled and analyzed 5 years (2008–2012) of cross-sectional STI morbidity data to identify multiple bacterial STI (chlamydia, gonorrhea, and syphilis) hot spot counties using hot spot analysis. Then, we examined the association between the multi-STI hot spots and select multiyear (2008–2012) sociodemographic factors (data obtained from the American Community Survey) using ordered spatial logistic regression analyses. Results Of the 2935 counties, ...
Source: Sexually Transmitted Diseases - Category: Sexual Medicine Tags: Original Study Source Type: research
Conclusions School-going indigenous adolescents in rural Panama have substantial STI burden. Targeted STI screening is required.
Source: Sexually Transmitted Diseases - Category: Sexual Medicine Tags: Original Studies Source Type: research
UCLA Health Rates of sexually transmitted diseases have risen for the past four years to record highs in the United States, according to the Centers for Disease Control and Prevention ’slatest analysis. In California, the state health departmentfound that the number of people diagnosed with syphilis, gonorrhea or chlamydia in 2017 was 45 percent higher than five years prior.These sorts of statistics may spark a fear that there ’s little we can do to protect ourselves — but that’s not the full story.Dr. Leena Nathan, an obstetrician/gynecologist at UCLA Health-Westlake Village, consults with peo...
Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news
myLAB Box, a healthcare company based in California, has developed and pioneered an at-home sexually transmitted disease (STD) testing service. With the number of STDs rising, the Centers for Disease Control and Prevention estimates that nearly 20 mi...
Source: Medgadget - Category: Medical Devices Authors: Tags: Exclusive Medicine Public Health Reproductive Medicine Source Type: blogs
This report presents baseline estimates of the use of these preventive services before the publication of QFP that can be used to monitor progress toward improving the quality of preventive care received by women and men of reproductive age. PERIOD COVERED: 2011-2013. DESCRIPTION OF THE SYSTEM: Three surveillance systems were used to document receipt of preventive health services among women and men of reproductive age as recommended in QFP. The National Survey of Family Growth (NSFG) collects data on factors that influence reproductive health in the United States since 1973, with a focus on fertility, sexual act...
Source: MMWR Surveill Summ - Category: Epidemiology Authors: Tags: MMWR Surveill Summ Source Type: research
Discussion Of the 30 different microbes which can be transmitted by sexual contact, 8 have the greatest incidence of transmitting disease. Four are curable (Chlamydia, Gonorrhea, Syphilis and Trichomoniasis) and 4 are incurable at present (Hepatitis B, Herpes simplex virus (HSV), Human immunodeficiency virus (HIV) and Human papillomavirus (HPV)). Sexually transmitted infections (STIs) are important as they can cause: Increased rates of acquisition of other STIs (ie HSV and syphilis increase the rate of HIV infection acquisition) Pelvic inflammatory disease and infertility Stillbirth and neonatal death Neonatal morbidity i...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
Conclusions: More than 25% of the population was infected with either agent. We successfully used in-house molecular methodologies for diagnosis and typing, showing HPV and C. trachomatis prevalence consistent to previous reports. Concomitant infections were found, HPV high-risk types were involved in half of these cases, representing a higher risk.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research
Recently I saw a young woman in my clinic for her annual exam. As usual, I asked her if she would like to be tested for sexually transmitted infections, and then we reviewed the “menu” of options: we could collect a swab of her cervix for chlamydia, gonorrhea, and trichomonas, and a PAP smear for human papillomavirus. We could collect blood for HIV, hepatitis C, syphilis, and herpes. We discussed the pros and cons and details of testing — not everyone wants every test. But she cheerfully consented to all of it, and when the results came back positive for chlamydia, she was shocked. “But I had no sym...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Infectious diseases Prevention Sex Sexual Conditions Source Type: blogs
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