Ex Vivo Lung Perfusion

This article will review the rationale for EVLP and the different EVLP methods including the Toronto EVLP method, describe technical details of EVLP, report on the clinical results of EVLP, and describe the use of EVLP as a platform to deliver different therapies.Recent FindingsEVLP has been demonstrated to be a safe method of assessing high-risk donor lungs. The long-term survival and graft function of patients that received high-risk donor lungs assessed and treated with EVLP have been shown to be comparable to those receiving conventional donor lungs. Preclinical studies demonstrate that EVLP can serve as a platform for the delivery of specifically targeted therapies to repair injured lungs.SummaryEVLP has demonstrated to be a promising tool for the assessment and recovery of injured donor lungs using pharmacologic agents as well as gene and cellular therapies.
Source: Current Transplantation Reports - Category: Transplant Surgery Source Type: research

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The application of mesenchymal stromal cell (MSC)-based therapy during ex vivo lung perfusion (EVLP) could repair injured donor lungs prior to transplantation. The aim of this study was to determine the efficacy of MSC therapy performed during EVLP on ischemia-reperfusion injury using a pig lung transplant model.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Tags: Original Pre-Clinical Science Source Type: research
In this study, we identify a link between members of the genus Veillonella and exercise performance. We observed an increase in Veillonella relative abundance in marathon runners postmarathon and isolated a strain of Veillonella atypica from stool samples. Inoculation of this strain into mice significantly increased exhaustive treadmill run time. Veillonella utilize lactate as their sole carbon source, which prompted us to perform a shotgun metagenomic analysis in a cohort of elite athletes, finding that every gene in a major pathway metabolizing lactate to propionate is at higher relative abundance postexercise. Us...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusions In both mouse and human, uNK cells are the most prominent immune cells that occupy the maternal-fetal interface. The uNK cells appear to engage and establish complex interactions with the surrounding tissue, which impact their function. As more cell subsets are identified within the heterogeneous uNK cell population, it is anticipated that their functional heterogeneity will extend beyond vascular modification, growth-promotion and memory generation. Author Contributions DS wrote the manuscript. LY provided the micrographs and WY edited the manuscript. Funding Work in the Yokoyama lab on uterine NK cells i...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusions Fibroblasts play a critical role during periodontal wound healing. These cell populations are needed for the regeneration of a stable fibrillar connection between the tooth root, the gingiva, and the periodontal ligament. Importantly, regeneration of connective tissues involves different cellular activities driven by fibroblasts populations. These include the secretion of matrix molecules and the organization of these matrix components into functionally active fibers that finally restore the periodontium. Future studies should explore the cellular and molecular regulation of these cell populations and gain a m...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
A 3-year-old male patient (blood type A+) with primary pulmonary vein stenosis (PVS) was listed for transplantation because of progressive PVS involving all 4 pulmonary veins with elevated right ventricular pressures and ongoing hemoptysis (Figure  1). Initial management included 3 cardiac catheterizations with balloon dilations, followed by sutureless repair of right pulmonary veins with simultaneous workup for lung transplantation (Ltx). The patient was listed for bilateral Ltx with recurrence of aggressive restenosis 2 months after surgi cal intervention, and balloon dilations were performed as a bridge to transplant.
Source: The Journal of Thoracic and Cardiovascular Surgery - Category: Cardiovascular & Thoracic Surgery Authors: Tags: Case Report Source Type: research
Giuseppe Ristagno1*, Francesca Fumagalli1, Barbara Bottazzi2, Alberto Mantovani2,3,4, Davide Olivari1, Deborah Novelli1 and Roberto Latini1 1Department of Cardiovascular Research, Mario Negri Institute for Pharmacological Research IRCCS, Milan, Italy 2Humanitas Clinical and Research Center-IRCCS, Milan, Italy 3Humanitas University, Milan, Italy 4The William Harvey Research Institute, Queen Mary University of London, London, United Kingdom The long pentraxin PTX3 is a member of the pentraxin family produced locally by stromal and myeloid cells in response to proinflammatory signals and microbial moieties. The p...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In this study the effect of NMP perfusion fluid on survival, metabolism and function of thawed cryopreserved human (h)MSC and porcine (p)MSC in suspension conditions was studied. Suspension conditions reduced the viability of pMSC by 40% in both perfusion fluid and culture medium. Viability of hMSC was reduced by suspension conditions by 15% in perfusion fluid, whilst no differences were found in survival in culture medium. Under adherent conditions, survival of the cells was not affected by perfusion fluid. The perfusion fluid did not affect survival of fresh MSC in suspension compared to the control culture medium. The f...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Ex vivo lung perfusion (EVLP) enables novel therapeutic approaches to repair damaged lungs for transplantation. We investigated the concept and feasibility of elective cell therapy for donor lungs. Dosing, efficacy and transgene expression kinetics of engineered mesenchymal stromal cell therapy were examined in pig EVLP and transplantation.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Tags: 8 Source Type: research
Cardiac transplantation is a life-saving intervention for advanced heart failure but it is limited by a shortage of suitable donor organs. Ex vivo heart perfusion (EVHP) represents a promising alternative for organ preservation and repair. We aimed to assess the utility of gene expression for better understanding and monitoring cardiac injury and repair during EVHP.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Tags: 433 Source Type: research
Lung transplantation is a lifesaving therapy for patients with end-stage lung disease, but donor shortage still represents a significant problem in the clinical practice. With the use of normothermic ex vivo lung perfusion (EVLP), the retrieved donor lungs are ventilated and perfused in an ex vivo circuit, allowing repair and extended assessment of marginal donor lungs before transplantation.1 Actual criteria used to evaluate lung suitability for transplantation during EVLP are lung function tests, radiographic and bronchoscopic findings and macroscopic assessment.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Tags: Original Clinical Science Source Type: research
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