Construction of ferrocene modified conducting polymer based amperometric urea biosensor

Publication date: Available online 7 April 2017 Source:Enzyme and Microbial Technology Author(s): Muamer Dervisevic, Esma Dervisevic, Mehmet Şenel, Emre Çevik, Huseyin Bekir Yildiz, Pınar Çamurlu Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor’s amperometric response was linear in the range of 0.1 to 8.5mM with the sensitivity of 0.54μA/mM, detection limit of 12μM, and short response time of 2s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insignificant interference.

Source: Enzyme and Microbial Technology - April 10, 2017 Category: Biotechnology Source Type: research