Identification of microbial communities, with a focus on foodborne pathogens, during kimchi manufacturing process using culture-independent and -dependent analyses

Publication date: August 2017 Source:LWT - Food Science and Technology, Volume 81 Author(s): Hae-Won Lee, So-Ra Yoon, Su-Ji Kim, Hee Min Lee, Jae Yong Lee, Ji-Hyun Lee, Sung Hyun Kim, Ji-Hyoung Ha Little is known about microbial communities associated with the kimchi manufacturing process. We investigated the structure of microbial communities, focusing on foodborne pathogens, such as Bacillus cereus, enterohemorrhagic Escherichia coli, Clostridium perfringens, Campylobacter jejuni, Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, and Yersinia enterocolitica, during the kimchi manufacturing process, including the brining, washing, and condiment stuffing phases, using culture-dependent and culture-independent analyses. Foodborne pathogens were not identified in any test sample by the culture-dependent analysis, whereas the culture-independent analysis targeting the hypervariable regions V3 and V4 of the 16S rRNA gene detected traces of general or potential foodborne pathogens, such as Campylobacter spp., Enterobacter spp., Escherichia or Shigella spp., Serratia spp., Vibrio spp., Aeromonas spp., Acinetobacter spp., Pseudomonas spp., and Enterococcus spp. However, as the kimchi manufacturing process proceeded, the structure of the microbial community changed, and potential foodborne pathogens were replaced by probiotics, such as lactic acid bacteria. Our results suggest that it may be worth utilizing the culture-independent analysis to complement the cu...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research