Phenotypic and molecular characterization of enteroaggregative Escherichia coli isolated in Kawasaki, Japan.

Phenotypic and molecular characterization of enteroaggregative Escherichia coli isolated in Kawasaki, Japan. Jpn J Infect Dis. 2017 Mar 28;: Authors: Kubomura A, Misaki T, Homma S, Matsuo C, Okabe N Abstract Enteroaggregative Escherichia coli (EAEC), an enteric pathogen, causes persistent diarrhea in children, HIV-infected individuals, and travelers in economically developing countries. However, the pathogenesis of EAEC infection is not well understood. The aim of this study is to characterize EAEC in Japan. During 2012-2014, we identified 40 EAEC strains carrying the aggR gene at the Kawasaki City Institute for Public Health, Japan. We characterized these strains using O:H-antigen typing, polymerase chain reaction (for pCVD432, astA, extended-spectrum beta-lactamase, and four aggregative adherence fimbriae genes), HEp-2 cell adherence, clump formation, and antimicrobial susceptibility testing. We were able to classify the 40 EAEC strains into 20 O:H types. Although specific O:H types are not correlated with HEp-2 cell AA, all the O99:H10, O131:H27, and O176:H34 EAEC strains which were most frequent O:H types detected in this study showed co-resistance to ampicillin, sulfamethoxazole-trimethoprim, and tetracycline. Based on results of adhesion assay and detection of virulence-related genes, no significant difference was found between asymptomatic and symptomatic cases. Irrespective of their origin, the possibility of virulence could ...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research