Non-contact luminescence lifetime cryothermometry for macromolecular crystallography

Temperature is a very important parameter when aiming to minimize radiation damage to biological samples during experiments that utilize intense ionizing radiation. A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long-wavelength X-rays. The temperature is derived from the temperature-dependent decay time constant of luminescence from a minuscule scintillation sensor (
Source: Journal of Synchrotron Radiation - Category: Physics Authors: Tags: cryogenic thermometry luminescence lifetime protein crystalography bismuth germanate (BGO) research papers Source Type: research

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A full-field coherent imaging approach suitable for hard X-rays based on a classical (i.e. Galilean) X-ray microscope is described. The method combines a series of low-resolution images acquired at different transverse lens positions into a single high-resolution image, overcoming the spatial resolution limit set by the numerical aperture of the objective lens. The optical principles of the approach are described, the successful reconstruction of simulated phantom data is demonstrated, and aspects of the reconstruction are discussed. The authors believe that this approach offers some potential benefits over conventional sc...
Source: Journal of Synchrotron Radiation - Category: Physics Authors: Tags: coherence imaging microscopy research papers Source Type: research
Conclusions: The findings indicate that hospitalizations are associated with a significant increase in mortality in patients recently diagnosed with HF. It is important to reinforce the need for the prevention of acute decompensated HF and for strategies to improve post-discharge outcomes. PMID: 31702737 [PubMed - in process]
Source: Archivos de Cardiologia de Mexico - Category: Cardiology Authors: Tags: Arch Cardiol Mex Source Type: research
ConclusionAmong the patients with VCD/VCF, the false negative rate among was 23.9% (17/71), but the missed cases were all minimal or mild grades. One or more VCFs were missed in 32.4% (23/71) of the cases with multiple VCFs. Appropriate severity grading was not reported for most cases.
Source: Journal of Orthopaedic Translation - Category: Orthopaedics Source Type: research
Publication date: Available online 8 November 2019Source: Neuroscience ResearchAuthor(s): Anggraeini Puspitasari, Hiroyuki Yamazaki, Hidemasa Kawamura, Takashi Nakano, Akihisa Takahashi, Tomoaki Shirao, Kathryn D HeldAbstractThe effects of X-irradiation on developing neurons and their functions are unclear. We used primary cultures of mouse hippocampal neurons to investigate the effects of X-irradiation on cell death in developing neurons by analyzing caspase-3, MAP2 and DAPI-labeled cells, and the phenotypes and function of surviving neurons, by examining GAD67-positive cells as a GABAergic marker, and the synaptic marker...
Source: Neuroscience Research - Category: Neuroscience Source Type: research
Authors: AlShazli ABAD, Amer AY, Sultan AM, Barakat AS, Koptan W, ElMiligui Y, Shaker H Abstract Study Design: This was a prospective case series of 30 patients with post-discectomy syndrome with an average of 18 months of follow-up (level IV). Purpose: The efficacy of post-discectomy syndrome managed by minimally invasive surgery transforaminal lumbar interbody fusion (MIS-TLIF) was evaluated. Overview of Literature: In post-discectomy syndrome wherein conservative treatment had failed, the best surgical treatment modality still remains controversial. Methods: Patients were functionally assessed using ...
Source: Asian Spine Journal - Category: Orthopaedics Tags: Asian Spine J Source Type: research
ConclusionsAlthough there is an expanding body of literature on AI and ML in cardiovascular medicine, the future these fields will have in clinical practice remains to be paved. In particular, there is a promising role in providing automated imaging interpretation, automated data extraction and quality control, and clinical risk prediction although these techniques require further refinement and evaluation.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Publication date: December 2019Source: Results in Physics, Volume 15Author(s): F. Akman, V. Turan, M.I. Sayyed, F. Akdemir, M.R. Kaçal, R. Durak, M.H.M. ZaidAbstractShielding parameter of eight (A–H) selected soil samples collected from different sites in Bingöl city and Genç district, Turkey have been comprehensively measured with fifteen different photon energies in the energy range from 13.94 keV to 88.04 keV. The soil samples were irradiated with 100 mCi 241Am, 10 mCi 133Ba and 40 mCi 109Cd radioactive point source using transmission geometry arrangement. The results were compared wit...
Source: Results in Physics - Category: Physics Source Type: research
Conclusions: Our results show that psychological factors are more strongly related to pain levels prior to non-invasive treatment in patients with osteoarthritis of the first carpometacarpal joint than patient characteristics and X-ray scores, which implies the important role of these factors in the reporting of symptoms. More research is needed to determine whether psychological factors will also affect treatment outcomes for patients treated non-invasively for osteoarthritis of the first carpometacarpal joint.IMPLICATIONS FOR REHABILITATIONPain is the most important complaint for patients with osteoarthritis of the first...
Source: Disability and Rehabilitation - Category: Rehabilitation Authors: Tags: Disabil Rehabil Source Type: research
Publication date: Available online 6 November 2019Source: Reproductive ToxicologyAuthor(s): Kai Craenen, Mieke Verslegers, Livine Craeghs, Roel Quintens, Ann Janssen, Amelie Coolkens, Sarah Baatout, Lieve Moons, Mohammed Abderrafi BenotmaneAbstractRecent studies highlighted a link between ionizing radiation exposure during neurulation and birth defects such as microphthalmos and anophthalmos. Because the mechanisms underlying these defects remain largely unexplored, we irradiated pregnant C57BL/6J mice (1.0 Gy, X-rays) at embryonic day (E)7.5, followed by histological and gene/protein expression analyses at defined days. I...
Source: Reproductive Toxicology - Category: Toxicology Source Type: research
Gina Hollenbeck said she did “everything right.” The Tennessee nurse and mother of two avoided cigarettes all her life. She ate organic food. She ran half marathons and played competitive tennis. But in 2015, she developed a persistent cough and rapidly lost weight. She didn’t have a primary-care doctor, so she consulted several specialists. For months, none could identify what was wrong. Finally, she paid out of pocket for chest X-rays and took them to an emergency room, where she was referred to a pulmonologist who diagnosed her with advanced lung cancer. “I was like, ‘This is a cruel joke,'...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Cancer Source Type: news
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