Non-contact luminescence lifetime cryothermometry for macromolecular crystallography

Temperature is a very important parameter when aiming to minimize radiation damage to biological samples during experiments that utilize intense ionizing radiation. A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long-wavelength X-rays. The temperature is derived from the temperature-dependent decay time constant of luminescence from a minuscule scintillation sensor (

Source: Journal of Synchrotron Radiation - April 4, 2017 Category: Physics Authors: Mykhaylyk, V.B. Wagner, A. Kraus, H. Tags: cryogenic thermometry luminescence lifetime protein crystalography bismuth germanate (BGO) research papers Source Type: research