The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle  orientation

Nature Cell Biology 19, 384 (2017). doi:10.1038/ncb3486 Authors: Justin Decarreau, Michael Wagenbach, Eric Lynch, Aaron R. Halpern, Joshua C. Vaughan, Justin Kollman & Linda Wordeman Microtubules tether centrosomes together during interphase. How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end-directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis.
Source: Nature Cell Biology - Category: Cytology Authors: Tags: Letter Source Type: research