Crystallization and preliminary X-ray crystallographic analysis of a bacterial Asn-transamidosome

In this study, the Asn-transamidosome from Pseudomonas aeruginosa was reconstructed and crystallized by mixing purified GatCAB complex, AspRS and tRNAAsn. The crystal of the Asn-transamidosome belonged to space group P21, with unit-cell parameters a = 93.3, b = 186.0, c = 287.8 Å, β = 93.3°, and diffracted to 3.73 Å resolution. Preliminary X-ray crystallographic analysis showed that the asymmetric unit contained two Asn-transamidosomes, each composed of two GatCABs, one AspRS dimer and two tRNAAsns, indicating that the construction of the current Asn-transamidosome differs from that of Thermus thermophilus.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: transamidosome aminoacyl-tRNA AspRS GatCAB Asn-tRNAAsn Pseudomonas aeruginosa crystallization communications Source Type: research
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