Crystallization and preliminary X-ray crystallographic studies of dehydroascorbate reductase (DHAR) from Oryza sativa L. japonica

In this study, recombinant OsDHAR protein was purified and crystallized using the sitting-drop vapour-diffusion method at pH 8.0 and 298 K. Plate-shaped crystals were obtained using 0.15 M potassium bromide, 30%(w/v) PEG MME 2000 as a precipitant, and the crystals diffracted to a resolution of 1.9 Å on beamline 5C at the Pohang Accelerator Laboratory. The X-ray diffraction data indicated that the crystal contained one OsDHAR molecule in the asymmetric unit and belonged to space group P21 with unit-cell parameters a = 47.03, b = 48.38, c = 51.83 Å, β = 107.41°.

Source: Acta Crystallographica Section F - May 24, 2014 Category: Biochemistry Authors: Do, H.Kim, I.-S.Kim, Y.-S.Shin, S.-Y.Kim, J.-J.Mok, J.-E.Park, S.-I.Wi, A.R.Park, H.Kim, H.-W.Yoon, H.-S.Lee, J.H. Tags: ascorbate dehydroascorbate reductase Oryza sativa L. japonica crystallization communications Source Type: research