Endo-symbiont mediated synthesis of gold nanobactericides and their activity against human pathogenic bacteria

Publication date: Available online 27 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Baker Syed, M.N. Nagendra Prasad, K. Mohan Kumar, B.L. Dhananjaya, Sreedharamurthy Satish Synthesis of gold nanobactericides (AuNBs) were achieved by treating 1mM chloroaurate with cell free supernatant of Aneurinibacillus migulanus. Formation of AuNBs was initially was monitored with change in color to ruby red. Further confirmation was assessed with UV-Visible spectra with maximum absorption occurring at 510nm. Transmission electron microscopy (TEM) analysis revealed the polydispersity of AuNBs with size distribution ranging from 10 to 60nm with an average size of 30nm. Crystalline nature was studied using X-Ray diffraction which exhibited characteristic peaks indexed to Bragg's reflection at 2θ angle which confers (111), (200), (220), and (311) planes suggesting AuNBs were face-centered cubic. Fourier transform infrared spectroscopy (FTIR) analysis revealed absorption peaks occurring at 3341cm−1, 1635cm−1 and 670cm−1 which corresponds to functional groups attributing to synthesis. The antibacterial efficacy of AuNBs were tested against selective human pathogenic bacteria and activity was measured as zone of inhibition by using disc and well diffusion. Bactericidal activity was interpreted with standard antibiotics gentamicin and kanamycin. Micro broth dilution assay expressed the minimal concentration of AuNBs to inhibit the growth of test pathogens. H...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research