Maternal Separation Diminishes Alpha-Adrenergic Receptors Density and Function in Renal Vasculature from male Wistar Kyoto rats.

Maternal Separation Diminishes Alpha-Adrenergic Receptors Density and Function in Renal Vasculature from male Wistar Kyoto rats. Am J Physiol Renal Physiol. 2017 Mar 22;:ajprenal.00591.2016 Authors: Loria AS, Osborn JL Abstract Adult rats exposed to maternal separation (MatSep) are normotensive but display lower glomerular filtration rate and increased renal neuroadrenergic drive. The aim of this study was to determine the renal alpha-adrenergic receptor density and the renal vascular responsiveness to adrenergic stimulation in male rats exposed to MatSep. In addition, baroreflex sensitivity was assessed to determine a component of neural control of the vasculature. Using tissue collected from 4-month-old MatSep and control rats, alpha 1-adrenergic receptors (α1-ARs) were measured in renal cortex and isolated renal vasculature using receptor binding assay, and the α-AR subtype gene expression was determined by RT-PCR. Renal cortical α1-AR density was similar between MatSep and control tissues (Bmax=44±1 vs. 42±2 fmol/mg protein, respectively); however, MatSep reduced α1-AR density in renal vasculature (Bmax=47±4 vs. 62±4 fmol/mg protein, p<0.05, respectively). In a separate group of rats, the pressor, bradycardic and renal vascular constrictor responses to acute norepinephrine injection (NE, 0.03-0.25 μg/μl) were determined in anesthetized rats. MatSep rats showed attenuated NE-induced renal vasoconstriction compared to ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research