Development and in-use evaluation of a novel Luminex MicroPlex microsphere-based (TRIOL) assay for simultaneous identification of Mycobacterium tuberculosis and detection of first-line and second-line anti-tuberculous drug resistance in China
Conclusions
The TRIOL assay has higher throughput, lower cost and is less labour intensive than the PCR-sequencing assays. The TRIOL assay is advantageous in having the capability to detect resistance to multiple drugs and an open-architecture system that allows addition of more specific primers to detect uncommon mutations. Inclusion of additional primers for the identification of non-tuberculous mycobacteria, spoligotyping and improvement of rifampicin resistance detection would enhance the use of the TRIOL assay in future clinical and epidemiological studies.
Source: Journal of Clinical Pathology - Category: Pathology Authors: Yin, F., Chan, J. F.-W., Zhu, Q., Fu, R., Chen, J. H.-K., Choi, G. K.-Y., Tee, K.-M., Li, L., Qian, S., Yam, W.-C., Lu, G., Yuen, K.-Y. Tags: Original article Source Type: research