Biofilm inhibition formation of clinical strains of Pseudomonas aeruginosa mutans, photocatalytic activity of azo dye and GC –MS analysis of leaves of Lagerstroemia speciosa

Publication date: Available online 9 March 2017 Source:Journal of Photochemistry and Photobiology B: Biology Author(s): V. Sai Saraswathi, Neethu Kamarudheen, K.V. Bhaskara Rao, K. Santhakumar The investigation was conducted to analyse the bioactive compounds from the leaf extracts of L. speciosa by GC–MS. The extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. The bioactive compounds from the leaves of L. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by Gas Chromatography–Mass Spectroscopy (GC–MS). The antibacterial activity was evaluated against clinical strain like Staphylococcus aureus, Escherichia coli, P. aeruginosa and Salmonella typhi by well diffusion technique. We also screened for antibacterial property against common food borne pathogens namely Listeria monocytogenes and Bacillus cereus at varied concentration 250μml−1 to 1000μml−1. Thereafter antibiofilm assay was carried out at from 250 to 1000μg/ml against P. aeruginosa (high biofilm forming pathogen) clinical strain by cover slip technique and the morphology of the pathogen was observed using Scanning Electron Microscopy-(SEM). It was observed that diverse class of secondary metabolites were found by GC–MS analysis for all the extracts upon the continuous extraction. It was found that only minimum inhibition was seen in alcoholic extract for antibacterial activity, whereas all ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech Therapy Source Type: research